Diagnostic anti-Bovine viral diarrhea virus (BVDV) antibodies pairs and antigens for animal health (animal infectious disease ) testing in ELISA test,competitive ELISA test, blocking ELISA test, Lateral flow immunoassay (LFIA), colloidal gold immunochromatographic assay, Chemiluminescent immunoassay (CLIA), turbidimetric inhibition immuno assay (TINIA), and immunonephelometry
Catalog Number: GMP-AD-Ruminants-16
Definition of the disease: Bovine viral diarrhea virus (BVDV) is a significant pathogen associated with gastrointestinal, respiratory, and reproductive diseases of cattle worldwide. It causes continuous economic losses to the cattle industry primarily due to decreased reproductive performance. The ability of virus to cross the placenta during early pregnancy can result in the birth of persistently infected (PI) calves. Persistently infected animals are generally much more efficient transmitters of BVDV than transiently or acutely infected animals because they are capable of shedding large quantities of virus throughout their lives and are considered the primary reservoirs for BVDV. Due to the nature of viral infections, there is no treatment to fully cure an animal of a viral infection. All control programs which are in use in many countries of the world, mainly depend upon the detection of PI animals, eliminating them and preventing their return into the herds. Detection of PI animals at early stage, particularly soon after birth is of significant benefit to implement BVDV control programs. Available diagnostic tests such as virus isolation (VI), immunohistochemistry (IHC), Antigen-Capture ELISA (ACE), and reverse transcriptase polymerase chain reaction (RT-PCR) are used for detection of PI cattle. Each method to detect BVDV has advantages, disadvantages, and applicability for different diagnostic situations. The reliability of diagnostic tests is optimized by choosing the appropriate sampling strategy on the basis of animal age.
Genemedi produces core animal health diagnostic ingredients-validated antibodies pairs Mouse anti-Bovine viral diarrhea virus (BVDV) monoclonal antibody and Bovine viral diarrhea virus (BVDV) antigens for rapid test kit of animal infectious disease with Bovine viral diarrhea virus (BVDV) to evaluate the animal health of Ruminants.
The paired antibodies are both monoclonal antibody(mab).
All the antibodies and antiges of animal disease test are suitable for in functional ELISA, and other immunoassays in dignostics.The antibody can act as a capture antibody and detection antibody. Antigens are validated as positive control materials.
Order information
Catalog No. (1~4, 4 antibodies in pairs) |
Size | Price(In USD) | Qty (Quantity) | Sum(In USD) |
---|---|---|---|---|
GMP-AD-Ruminants-16Ab-1 | Size:1mg | 3090 | ||
GMP-AD-Ruminants-16Ab-1 | Size:10mg | 21935 | ||
GMP-AD-Ruminants-16Ab-1 | Size:100mg | 148000 | ||
GMP-AD-Ruminants-16Ab-2 | Size:1mg | 3090 | ||
GMP-AD-Ruminants-16Ab-2 | Size:10mg | 21935 | ||
GMP-AD-Ruminants-16Ab-2 | Size:100mg | 148000 | ||
GMP-AD-Ruminants-16Ab-3 | Size:1mg | 3090 | ||
GMP-AD-Ruminants-16Ab-3 | Size:10mg | 21935 | ||
GMP-AD-Ruminants-16Ab-3 | Size:100mg | 148000 | ||
GMP-AD-Ruminants-16Ab-4 | Size:1mg | 3090 | ||
GMP-AD-Ruminants-16Ab-4 | Size:10mg | 21935 | ||
GMP-AD-Ruminants-16Ab-4 | Size:100mg | 148000 | ||
GMP-AD-Ruminants-16Ag-1 | Size:1mg | 3090 | ||
GMP-AD-Ruminants-16Ag-1 | Size:10mg | 21935 | ||
GMP-AD-Ruminants-16Ag-1 | Size:100mg | 148000 | ||
GMP-AD-Ruminants-16Ag-2 | Size:1mg | 3090 | ||
GMP-AD-Ruminants-16Ag-2 | Size:10mg | 21935 | ||
GMP-AD-Ruminants-16Ag-2 | Size:100mg | 148000 | ||
Shipping Cost: | 760.00 | |||
Total: | ||||
Description
GMP-AD-Ruminants-16Ab, GMP-AD-Ruminants-16Ag
Cat No. | GMP-AD-Ruminants-16Ab |
Antigens | Bovine viral diarrhea virus (BVDV) |
Antibody | Mouse anti-Bovine viral diarrhea virus (BVDV) monoclonal antibody |
Resource (expression host) | hybridoma |
Specics/Isotypes | Mouse IgG |
Bioactivity validation | Antibody Binding, Immunogen in Sandwich Elisa, lateral-flow tests,and other immunoassays in Bovine viral diarrhea virus (BVDV) level test and Ruminants-diagnositcs. |
Antigen description | Monoclonal antibodies reactive to the bovine viral diarrhea virus (BVDV) protein gp53 were produced and characterized. These antibodies and our panel of anti-p80/125 monoclonal antibodies were tested for their cross-reactivity with 11 different North American and European (Danish) BVDV strains and isolates including viruses of both cytopathic and noncytopathic biotypes. The four anti-gp53 monoclonal antibodies were neutralizing for the homologous Danish cytopathic isolate and cross-reacted with all BVDV strains examined except for the Draper strain. Further, anti-gp53 monoclonal antibodies neutralized the majority of BVDV strains examined. The anti-p80/125 monoclonal antibodies cross-reacted with all eleven strains and isolates tested. This indicated that various strains of BVDV have common epitopes. The broad cross-reactivities demonstrated by these monoclonal antibodies suggest that a pool of these antibodies may be used for detection of BVDV cellular contamination or for virus isolation, in place of polyclonal antiserum. |
Purity | Purity: ≥95% (SDS-PAGE) |
Application | Paired antibody immunoassay validation in Sandwich ELISA, Lateral flow immunoassay (LFIA), and other immunoassays; |
Formulation | Lyophilized from sterile PBS, PH 7.4 |
Storage | Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles. |
Cat No. | GMP-AD-Ruminants-16Ag |
Antigens | Bovine viral diarrhea virus (BVDV) |
Resource (expression host) | E.coli |
Specics/Isotypes | Bovine viral diarrhea virus (BVDV) |
Bioactivity validation | Antibody Binding, Immunogen in Sandwich Elisa, lateral-flow tests,and other immunoassays in Bovine viral diarrhea virus (BVDV) level test and Ruminants-diagnositcs. |
Tag | His |
Antigen description | Monoclonal antibodies reactive to the bovine viral diarrhea virus (BVDV) protein gp53 were produced and characterized. These antibodies and our panel of anti-p80/125 monoclonal antibodies were tested for their cross-reactivity with 11 different North American and European (Danish) BVDV strains and isolates including viruses of both cytopathic and noncytopathic biotypes. The four anti-gp53 monoclonal antibodies were neutralizing for the homologous Danish cytopathic isolate and cross-reacted with all BVDV strains examined except for the Draper strain. Further, anti-gp53 monoclonal antibodies neutralized the majority of BVDV strains examined. The anti-p80/125 monoclonal antibodies cross-reacted with all eleven strains and isolates tested. This indicated that various strains of BVDV have common epitopes. The broad cross-reactivities demonstrated by these monoclonal antibodies suggest that a pool of these antibodies may be used for detection of BVDV cellular contamination or for virus isolation, in place of polyclonal antiserum. |
Purity | Purity: ≥95% (SDS-PAGE) |
Application | Paired antibody immunoassay validation in Sandwich ELISA, Lateral flow immunoassay (LFIA), and other immunoassays; |
Formulation | Lyophilized from sterile PBS, PH 7.4 |
Storage | Store at -20℃ to -165℃ under sterile conditions. Avoid repeated freeze-thaw cycles. |
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