Chlamydophila spp. antibody and antigen (recombinant protein)

Diagnostic anti-Chlamydophila spp. antibodies pairs and antigen for animal health (animal Cat/Feline infectious disease respiratory disease) testing in ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA and POCT

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Product information

Catalog No. Description US $ Price (per mg)
GMP-VT-P003-Ag01 Recombinant Chlamydophila spp. protein $3090.00
GMP-VT-P003-Ab01 Anti-Chlamydophila spp. mouse monoclonal antibody (mAb) $3090.00
GMP-VT-P003-Ab02 Anti-Chlamydophila spp. mouse monoclonal antibody (mAb) $3090.00

Size: 1mg | 10mg | 100mg



Product Description

Cat No. GMP-VT-P003-Ag01
Product Name Recombinant Chlamydophila spp. protein
Pathogen Chlamydophila spp.
Expression platform E.coli
Isotypes Recombinant Antigen
Bioactivity validation Anti-Chlamydophila spp. antibodies binding, Immunogen in Sandwich Elisa, lateral-flow tests, and other immunoassays as control material in Chlamydophila spp. level test of animal Cat/Feline infectious disease with respiratory disease.
Tag His
Product description Recombinant Chlamydophila spp. proteinwas expressed in E.coli - based prokaryotic cell expression system and is expressed with 6 HIS tag at the C-terminus.
Purity Purity: ≥95% (SDS-PAGE)
Application Paired antibody immunoassay validation in Sandwich ELISA, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation Lyophilized from sterile PBS, PH 7.4
Storage Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.


Cat No. GMP-VT-P003-Ab01,GMP-VT-P003-Ab02
Pathogen Chlamydophila spp.
Product Name Anti-Chlamydophila spp. mouse monoclonal antibody (mAb)
Expression platform CHO
Isotypes Mouse IgG
Bioactivity validation Recombinant Chlamydophila spp. antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Chlamydophila spp. antibodies in Chlamydophila spp. level test of animal Cat/Feline infectious disease with respiratory disease.
Product description Anti-Chlamydophila spp. mouse monoclonal antibody (mAb) is a mouse monoclonal antibody produced by CHO technology. The antibody is ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Chlamydophila spp. antibodies./td>
Purity Purity: ≥95% (SDS-PAGE)
Application Paired antibody immunoassay validation in Sandwich ELISA, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation Lyophilized from sterile PBS, PH 7.4
Storage Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.


Reference




    Validation Data


    Click to get more Data / Case study about the product.



    Pathogen


    Cat/Feline Chlamydophila spp.: Unveiling an Intricate Pathogen

    Introduction

    In the realm of veterinary medicine, the microscopic world often hides formidable adversaries. Among these, Cat/Feline Chlamydophila spp. stands as an enigmatic yet significant pathogen. In this comprehensive exploration, we delve into the depths of its taxonomy, structural intricacies, host interactions, associated diseases, and diagnostic methods. By the end, you will gain a profound understanding of this fascinating obligate intracellular bacterium.

    1. Taxonomic Nomenclature and Essence

    Cat/Feline Chlamydophila spp. is the collective term for a group of obligate intracellular bacteria that primarily target domestic cats (Felis catus). Within the realm of taxonomy, they belong to the family Chlamydiaceae and constitute a unique entity. These microorganisms, while classified as bacteria, bear Gram-negative characteristics, setting them apart from other prokaryotic organisms.

    2. Unpacking the Intricacies of Chlamydophila

    At its core, Cat/Feline Chlamydophila spp. represents prokaryotic life forms. This classification places them among the bacteria, distinct from eukaryotic organisms and viruses. Unlike free-living bacteria, they are obligate intracellular parasites, relying on host cells for their replication and survival.

    3. Structural Aspects: Genes and Proteins

    At the molecular level, Chlamydophila spp. are characterized by specific genetic and proteinaceous components. The 16S ribosomal RNA gene (16S rRNA) serves as a pivotal genetic marker frequently employed in phylogenetic analyses. This gene offers insights into the evolutionary relationships among different Chlamydia species, aiding in their classification and identification.

    The pathogen's survival and infectivity are closely linked to its suite of proteins. Among these, the type III secretion system proteins play a pivotal role. These intricate machinery components facilitate the translocation of effector proteins into the host cell, enabling Chlamydophila spp. to establish their intracellular niche.

    4. Host Range and Consequences: Diseases

    The primary host targeted by Cat/Feline Chlamydophila spp. is the domestic cat. These bacteria are responsible for causing a disease known as feline chlamydiosis, clinically referred to as feline chlamydophilosis. This ailment predominantly manifests in the upper respiratory tract of infected cats, unleashing symptoms such as sneezing, coughing, nasal discharge, and conjunctivitis. In severe cases, it can progress to pneumonia, posing a significant health threat to feline populations.

    5. Unlocking the Diagnostic Arsenal

    Effectively combating Cat/Feline Chlamydophila spp. infections relies on precise diagnostic methods. Several techniques have been developed to detect and identify the presence of these pathogens:

    Polymerase Chain Reaction (PCR): PCR assays are instrumental in amplifying and identifying specific DNA sequences associated with Chlamydophila spp. These assays often target genes such as the 16S rRNA gene or the outer membrane protein A (ompA) gene. PCR amplification enables the detection of even trace amounts of Chlamydophila DNA.

    Enzyme-Linked Immunosorbent Assay (ELISA): ELISA tests capitalize on the host's immune response by detecting antibodies directed against chlamydial antigens. One of the primary targets for detection is the major outer membrane protein (MOMP). ELISA assays are highly sensitive and can provide insights into the stage of infection.

    Nucleic Acid Hybridization: This technique employs specific probes designed to bind to Chlamydophila spp. DNA or RNA sequences. By utilizing complementary base pairing, it allows for the detection of genetic material specific to the pathogen.

    Immunofluorescence Assay (IFA): IFA is a powerful method for detecting chlamydial antigens within clinical samples. It involves labeling antibodies with fluorescent markers, which bind to chlamydial antigens, making them visible under fluorescent microscopy.

    Cultural Methods: Although less commonly employed due to its technical demands, Chlamydophila spp. can be cultured under controlled laboratory conditions. This method involves the isolation and propagation of the pathogen in specific cell lines, which can then be visualized and confirmed through microscopy.

    Conclusion

    In the intricate world of veterinary microbiology, Cat/Feline Chlamydophila spp. stands as a captivating example of adaptation and host-pathogen interaction. Its obligate intracellular lifestyle, genetic markers, protein machinery, and clinical manifestations make it a focal point of research and diagnostic efforts. By gaining a deeper understanding of this pathogen, veterinarians and researchers can better combat feline chlamydiosis and contribute to the well-being of our feline companions.



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