Rickettsia rickettsii antibody and antigen (recombinant protein)

Diagnostic anti-Rickettsia rickettsii antibodies pairs and antigen for animal health (animal Dog/Canine infectious disease Rocky Mountain spotted fever) testing in ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA and POCT

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Product information

Catalog No. Description US $ Price (per mg)
GMP-VT-P039-Ag01 Recombinant Rickettsia rickettsii protein $3090.00
GMP-VT-P039-Ab01 Anti-Rickettsia rickettsii mouse monoclonal antibody (mAb) $3090.00
GMP-VT-P039-Ab02 Anti-Rickettsia rickettsii mouse monoclonal antibody (mAb) $3090.00

Size: 1mg | 10mg | 100mg



Product Description

Cat No. GMP-VT-P039-Ag01
Product Name Recombinant Rickettsia rickettsii protein
Pathogen Rickettsia rickettsii
Expression platform E.coli
Isotypes Recombinant Antigen
Bioactivity validation Anti-Rickettsia rickettsii antibodies binding, Immunogen in Sandwich Elisa, lateral-flow tests, and other immunoassays as control material in Rickettsia rickettsii level test of animal Dog/Canine infectious disease with Rocky Mountain spotted fever.
Tag His
Product description Recombinant Rickettsia rickettsii proteinwas expressed in E.coli - based prokaryotic cell expression system and is expressed with 6 HIS tag at the C-terminus.
Purity Purity: ≥95% (SDS-PAGE)
Application Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation Lyophilized from sterile PBS, PH 7.4
Storage Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.


Cat No. GMP-VT-P039-Ab01,GMP-VT-P039-Ab02
Pathogen Rickettsia rickettsii
Product Name Anti-Rickettsia rickettsii mouse monoclonal antibody (mAb)
Expression platform CHO
Isotypes Mouse IgG
Bioactivity validation Recombinant Rickettsia rickettsii antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Rickettsia rickettsii antibodies in Rickettsia rickettsii level test of animal Dog/Canine infectious disease with Rocky Mountain spotted fever.
Product description Anti-Rickettsia rickettsii mouse monoclonal antibody (mAb) is a mouse monoclonal antibody produced by CHO technology. The antibody is ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Rickettsia rickettsii antibodies./td>
Purity Purity: ≥95% (SDS-PAGE)
Application Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation Lyophilized from sterile PBS, PH 7.4
Storage Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.


Reference




    Validation Data


    Click to get more Data / Case study about the product.



    Pathogen


    Rickettsia rickettsii, a gram-negative, obligate intracellular bacterium, is the primary causative agent of Rocky Mountain spotted fever (RMSF). This pathogen belongs to the family Rickettsiaceae, order Rickettsiales, and genus Rickettsia. It is primarily transmitted through tick bites, particularly by Dermacentor species ticks.

    Rickettsia rickettsii has a unique structural organization that enables its survival within host cells. The bacterium possesses a complex cell envelope consisting of both an outer membrane and inner membranes. The outer membrane contains various proteins, including the rickettsial outer membrane protein A (romA), which plays a crucial role in bacterial invasion and intracellular survival. This protein aids in adhesion to host cells and facilitates the uptake of the bacterium into the cytoplasm.

    The genome of Rickettsia rickettsii is approximately 1.2 million base pairs in size and encodes numerous genes involved in various cellular functions. These genes include those responsible for metabolic pathways, DNA replication, protein synthesis, and virulence factors. Notably, the bacterium harbors a large number of genes related to transport systems, allowing it to scavenge essential nutrients from the host cell environment.

    Rickettsia rickettsii primarily infects humans but can also affect certain mammals. In humans, infection typically occurs following a tick bite. Once in the bloodstream, the bacterium disseminates to various organs, including the endothelial cells lining blood vessels, leading to vasculitis and subsequent tissue damage. This damage manifests clinically as RMSF, characterized by symptoms such as high fever, severe headache, muscle pain, rash (typically starting on the wrists and ankles and spreading inward), and, in severe cases, organ dysfunction. RMSF can be fatal if not promptly diagnosed and treated.

    Aside from Rocky Mountain spotted fever, Rickettsia rickettsii is associated with similar diseases in different regions. In Brazil, it causes Brazilian spotted fever (BSF), while in Mexico, it leads to Mexican spotted fever (MSF). These diseases share many clinical manifestations with RMSF, including fever, rash, and systemic complications. Similarly, Indian tick typhus, caused by Rickettsia rickettsii or closely related strains, presents with fever, rash, and other systemic symptoms.

    Accurate and timely diagnosis of Rickettsia rickettsii infection is crucial for effective management. Serological techniques are commonly employed for diagnostic testing. These methods detect the presence of antibodies produced by the host immune system in response to the pathogen. Enzyme-linked immunosorbent assay (ELISA) and immunofluorescence assay (IFA) are widely used serological tests, allowing the detection and quantification of specific antibodies.

    Molecular diagnostic methods, such as polymerase chain reaction (PCR) and other nucleic acid amplification tests (NAATs), offer enhanced sensitivity and specificity. These techniques target specific genes or genetic regions unique to Rickettsia rickettsii, enabling the detection of the bacterium's DNA in clinical samples. Commonly targeted genes include gltA (citrate synthase gene), ompA and ompB (outer membrane protein genes), and the 17kDa antigen gene.

    In conclusion, Rickettsia rickettsii is an intracellular bacterium responsible for Rocky Mountain spotted fever and related diseases. Its unique structure and genetic composition contribute to its ability to invade and survive within host cells. Accurate diagnosis of Rickettsia rickettsii infection relies on a combination of serological and molecular methods, enabling prompt treatment and improved patient outcomes.



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