Mycobacterium avium subsp. paratuberculosis antibody and antigen (recombinant protein)

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Diagnostic anti-Mycobacterium avium subsp. paratuberculosis antibodies pairs and antigen for animal health (animal Bovines/Cattle, Ovines/Sheep, Caprine/Goat infectious disease paratuberculosis) testing in ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA and POCT

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Product information

Catalog No.	Description	US $ Price (per mg)
GMP-VT-P048-Tg001-Ag01	Recombinant Mycobacterium avium subsp. paratuberculosis protein	$3090.00
GMP-VT-P048-Tg001-Ab01	Anti-Mycobacterium avium subsp. paratuberculosis mouse monoclonal antibody (mAb)	$3090.00
GMP-VT-P048-Tg001-Ab02	Anti-Mycobacterium avium subsp. paratuberculosis mouse monoclonal antibody (mAb)	$3090.00
GMP-VT-P048-Tg001-Ab03	Anti-Mycobacterium avium subsp. paratuberculosis human monoclonal antibody (mAb)	$3090.00
GMP-VT-P048-Tg001-Ab04	Anti-Mycobacterium avium subsp. paratuberculosis human monoclonal antibody (mAb)	$3090.00

Size： 1mg | 10mg | 100mg

Product Description

Cat No.	GMP-VT-P048-Tg001-Ag01
Product Name	Recombinant Mycobacterium avium subsp. paratuberculosis protein
Pathogen	Mycobacterium avium subsp. paratuberculosis
Expression platform	E.coli
Isotypes	Recombinant Antigen
Bioactivity validation	Anti-Mycobacterium avium subsp. paratuberculosis antibodies binding, Immunogen in Sandwich Elisa, lateral-flow tests, and other immunoassays as control material in Mycobacterium avium subsp. paratuberculosis level test of animal Bovines/Cattle, Ovines/Sheep, Caprine/Goat infectious disease with paratuberculosis.
Tag	His
Product description	Recombinant Mycobacterium avium subsp. paratuberculosis proteinwas expressed in E.coli - based prokaryotic cell expression system and is expressed with 6 HIS tag at the C-terminus.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from sterile PBS, PH 7.4
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Cat No.	GMP-VT-P048-Tg001-Ab01,GMP-VT-P048-Tg001-Ab02
Pathogen	Mycobacterium avium subsp. paratuberculosis
Product Name	Anti-Mycobacterium avium subsp. paratuberculosis mouse monoclonal antibody (mAb)
Expression platform	CHO
Isotypes	Mouse IgG
Bioactivity validation	Recombinant Mycobacterium avium subsp. paratuberculosis antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Mycobacterium avium subsp. paratuberculosis antibodies in Mycobacterium avium subsp. paratuberculosis level test of animal Bovines/Cattle, Ovines/Sheep, Caprine/Goat infectious disease with paratuberculosis.
Product description	Anti-Mycobacterium avium subsp. paratuberculosis mouse monoclonal antibody (mAb) is a mouse monoclonal antibody produced by CHO technology. The antibody is ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Mycobacterium avium subsp. paratuberculosis antibodies.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from sterile PBS, PH 7.4
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Cat No.	GMP-VT-P048-Tg001-Ab03,GMP-VT-P048-Tg001-Ab04
Pathogen	Mycobacterium avium subsp. paratuberculosis
Product Name	Anti-Mycobacterium avium subsp. paratuberculosis human monoclonal antibody (mAb)
Expression platform	CHO
Isotypes	Human lgG1
Bioactivity validation	Recombinant Mycobacterium avium subsp. paratuberculosis antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Mycobacterium avium subsp. paratuberculosis antibodies in Mycobacterium avium subsp. paratuberculosis level test of animal Bovines/Cattle, Ovines/Sheep, Caprine/Goat infectious disease with paratuberculosis.
Product description	Anti-Mycobacterium avium subsp. paratuberculosis mouse monoclonal antibody (mAb) is a human monoclonal antibody produced by CHO. The antibody is ELISA validated as capture antibody and detection antibody pair.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from sterile PBS, PH 7.4
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Reference

Validation Data

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Pathogen Information

MAP is a slow-growing bacterium that possesses a unique cell wall structure consisting of multiple layers of peptidoglycan, arabinogalactan, and mycolic acids. This complex structure contributes to the bacterium's resistance to disinfectants, antibiotics, and host immune defenses. MAP also expresses several genes and proteins essential for its virulence, such as the IS900 gene, a specific marker for MAP.

The transmission of MAP occurs primarily through the fecal-oral route, with ingestion of contaminated feed, water, or milk being the most common mode of transmission. Infection can also occur in utero or during the neonatal phase, with calves being the most susceptible to infection.

Several diagnostic methods are available for detecting MAP in animals suspected of Johne's disease. Culture-based techniques, such as acid-fast bacilli (AFB) staining and bacterial culture, have long been considered the gold standard for MAP detection. However, these methods are time-consuming, laborious, and have limitations in sensitivity and specificity, especially during the subclinical phase of infection.

Nucleic acid amplification tests (NAATs), such as polymerase chain reaction (PCR), have emerged as rapid and sensitive alternatives for MAP detection. PCR-based assays target specific regions of the MAP genome, including the IS900 gene, which is unique to MAP. Other genes, such as F57, have also been used for PCR-based detection. NAATs can detect MAP DNA in fecal, milk, or tissue samples, and have been shown to be more sensitive than culture-based methods.

Serological assays, such as enzyme-linked immunosorbent assay (ELISA), detect host antibodies against MAP-specific antigens, including Hsp70 and antigen 85 complex proteins. ELISA has advantages over culture-based methods and NAATs in terms of speed, ease of use, and cost-effectiveness. However, serological assays have limitations in terms of specificity, as cross-reactivity with other mycobacteria can occur, and sensitivity, especially during the early stages of infection.

A combination of different diagnostic methods may be necessary for accurate diagnosis, depending on the stage of infection and the animal species. For example, PCR-based assays may be more suitable for detecting subclinical infections, while ELISA may be more suitable for identifying animals with active disease.

Prevention and control of Johne's disease rely on implementing herd management practices that reduce the risk of transmission and infection. These practices include maintaining good hygiene in animal housing and feeding areas, using pathogen-free feed and water sources, and culling infected animals. Vaccination against MAP has been developed and used in some countries, but its effectiveness varies and further research is needed to improve its efficacy.

In conclusion, Mycobacterium avium subsp. paratuberculosis is a bacterial pathogen that causes Johne's disease, a chronic infectious disease affecting ruminant animals. Diagnostic methods for detecting MAP have evolved over the years, with NAATs and serological assays now being widely used. Preventing and controlling Johne's disease requires implementing effective herd management practices, including vaccination where appropriate.

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