Bovine Herpes Virus 2 antibody and antigen (recombinant protein)

Diagnostic anti-Bovine Herpes Virus 2 antibodies pairs and antigen for animal health (animal Bovines/Cattle infectious disease infectious bovine rhinotracheitis, abortion, infectious pustular vulvovaginitis, and systemic infection in neonates) testing in ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA and POCT

Target products collectionGo to Ruminants disease testing products collection >>


Product information

Catalog No. Description US $ Price (per mg)
GMP-VT-P065-Ag01 Recombinant Bovine Herpes Virus 2 protein $3090.00
GMP-VT-P065-Ab01 Anti-Bovine Herpes Virus 2 mouse monoclonal antibody (mAb) $3090.00
GMP-VT-P065-Ab02 Anti-Bovine Herpes Virus 2 mouse monoclonal antibody (mAb) $3090.00

Size: 1mg | 10mg | 100mg



Product Description

Cat No. GMP-VT-P065-Ag01
Product Name Recombinant Bovine Herpes Virus 2 protein
Pathogen Bovine Herpes Virus 2
Expression platform E.coli
Isotypes Recombinant Antigen
Bioactivity validation Anti-Bovine Herpes Virus 2 antibodies binding, Immunogen in Sandwich Elisa, lateral-flow tests, and other immunoassays as control material in Bovine Herpes Virus 2 level test of animal Bovines/Cattle infectious disease with infectious bovine rhinotracheitis, abortion, infectious pustular vulvovaginitis, and systemic infection in neonates.
Tag His
Product description Recombinant Bovine Herpes Virus 2 proteinwas expressed in E.coli - based prokaryotic cell expression system and is expressed with 6 HIS tag at the C-terminus.
Purity Purity: ≥95% (SDS-PAGE)
Application Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation Lyophilized from sterile PBS, PH 7.4
Storage Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.


Cat No. GMP-VT-P065-Ab01,GMP-VT-P065-Ab02
Pathogen Bovine Herpes Virus 2
Product Name Anti-Bovine Herpes Virus 2 mouse monoclonal antibody (mAb)
Expression platform CHO
Isotypes Mouse IgG
Bioactivity validation Recombinant Bovine Herpes Virus 2 antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Bovine Herpes Virus 2 antibodies in Bovine Herpes Virus 2 level test of animal Bovines/Cattle infectious disease with infectious bovine rhinotracheitis, abortion, infectious pustular vulvovaginitis, and systemic infection in neonates.
Product description Anti-Bovine Herpes Virus 2 mouse monoclonal antibody (mAb) is a mouse monoclonal antibody produced by CHO technology. The antibody is ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Bovine Herpes Virus 2 antibodies./td>
Purity Purity: ≥95% (SDS-PAGE)
Application Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation Lyophilized from sterile PBS, PH 7.4
Storage Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.


Reference




    Validation Data


    Click to get more Data / Case study about the product.



    Pathogen


    Escherichia coli K99: A Comprehensive Overview

    Introduction

    Escherichia coli, commonly abbreviated as E. coli, is a ubiquitous bacterium found in the gastrointestinal tracts of humans and warm-blooded animals. Among the diverse strains of E. coli, one of particular interest is Escherichia coli K99, known for its capacity to induce gastrointestinal infections, notably among neonatal calves. This extended discourse aims to provide an in-depth understanding of E. coli K99, its classification, structure, host specificity, associated diseases, and diagnostic methods.

    Classification

    Escherichia coli is classified as a Gram-negative bacterium within the Enterobacteriaceae family. As a prokaryotic microorganism, it lacks a well-defined nucleus and membrane-bound organelles. E. coli is a member of the Enterobacteriaceae family, which encompasses a wide array of Gram-negative bacteria, many of which are pathogenic.

    Pathogen Structure

    One of the distinguishing features of Escherichia coli K99 is the presence of specialized surface structures known as fimbriae or pili. In this context, the "F5" gene is pivotal, as it encodes the K99 fimbrial adhesin protein. This protein is crucial for the bacterium's ability to adhere to the host's intestinal lining and initiate infection. The K99 fimbriae are elongated appendages that facilitate adhesion to specific receptors on the host cell surface.

    Hosts Infected and Associated Diseases

    Escherichia coli K99 primarily targets young calves, making it a significant concern in the agricultural industry. The infection caused by E. coli K99 in neonatal calves is commonly referred to as "neonatal calf diarrhea" or "scours." This condition results in severe and often life-threatening diarrhea in young calves. The clinical manifestations include dehydration, electrolyte imbalances, and a substantial economic burden on livestock operations due to calf mortality and decreased growth rates.

    Pathogenesis

    The pathogenesis of Escherichia coli K99 involves a complex interplay of virulence factors. The K99 fimbrial adhesin protein plays a central role by promoting attachment to the host's intestinal epithelium. Once attached, E. coli K99 can colonize the host's gut, leading to the development of diarrhea. Furthermore, the bacterium can produce various toxins, such as enterotoxins, which further contribute to the severity of the disease by altering the host's intestinal function.

    Diagnostic Methods

    Accurate and timely diagnosis of Escherichia coli K99 infections is essential for effective disease management and prevention. Several diagnostic methods are employed, including:

    Nucleic Acid-Based Methods: Polymerase Chain Reaction (PCR) is a common technique used to detect E. coli K99. PCR assays target specific genes, such as the K99 fimbrial gene (F5) and the 16S ribosomal RNA gene, which is a conserved region in bacterial genomes.

    Cultural Techniques: Isolation and culturing of the bacterium from clinical samples can confirm the presence of E. coli K99. This method, though time-consuming, provides definitive results.

    Serological Tests: Serological tests, including enzyme-linked immunosorbent assays (ELISA), can detect antibodies produced by the host in response to E. coli K99 infection.

    Prevention and Control

    Preventing Escherichia coli K99 infections in calves is a multifaceted challenge. Strategies may include:

    Vaccination: The development of vaccines targeting E. coli K99 has been explored as a preventive measure.

    Hygiene and Sanitation: Maintaining clean and hygienic conditions in calf-rearing facilities can reduce the risk of infection.

    Probiotics and Prebiotics: These can be used to promote a healthy gut microbiota in calves, potentially reducing susceptibility to E. coli K99.



    About GDU


    GDU

    GDU helps global diagnostic partners in high quality of raw material discovery, development, and application. GDU believes in Protein&antibody Innovation for more reliable diagnostic solutions.