Hypoderma bovis and H. lineatum Hypodermins A antibody and antigen (recombinant protein)

 Download 

Diagnostic anti-Hypoderma bovis and H. lineatum Hypodermins A antibodies pairs and antigen for animal health (animal Bovines/Cattle infectious disease Hypodermiasis) testing in ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA and POCT

Go to Ruminants disease testing products collection >>

Product information

Catalog No.	Description	US $ Price (per mg)
GMP-VT-P080-Tg001-Ag01	Recombinant Hypoderma bovis and H. lineatum Hypodermins A protein	$3090.00
GMP-VT-P080-Tg001-Ab01	Anti-Hypoderma bovis and H. lineatum Hypodermins A mouse monoclonal antibody (mAb)	$3090.00
GMP-VT-P080-Tg001-Ab02	Anti-Hypoderma bovis and H. lineatum Hypodermins A mouse monoclonal antibody (mAb)	$3090.00
GMP-VT-P080-Tg001-Ab03	Anti-Hypoderma bovis and H. lineatum Hypodermins A human monoclonal antibody (mAb)	$3090.00
GMP-VT-P080-Tg001-Ab04	Anti-Hypoderma bovis and H. lineatum Hypodermins A human monoclonal antibody (mAb)	$3090.00

Size： 1mg | 10mg | 100mg

Product Description

Cat No.	GMP-VT-P080-Tg001-Ag01
Product Name	Recombinant Hypoderma bovis and H. lineatum Hypodermins A protein
Pathogen	Hypoderma bovis and H. lineatum
Expression platform	E.coli
Isotypes	Recombinant Antigen
Bioactivity validation	Anti-Hypoderma bovis and H. lineatum Hypodermins A antibodies binding, Immunogen in Sandwich Elisa, lateral-flow tests, and other immunoassays as control material in Hypoderma bovis and H. lineatum level test of animal Bovines/Cattle infectious disease with Hypodermiasis.
Tag	His
Product description	Recombinant Hypoderma bovis and H. lineatum Hypodermins A proteinwas expressed in E.coli - based prokaryotic cell expression system and is expressed with 6 HIS tag at the C-terminus.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from sterile PBS, PH 7.4
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Cat No.	GMP-VT-P080-Tg001-Ab01,GMP-VT-P080-Tg001-Ab02
Pathogen	Hypoderma bovis and H. lineatum
Product Name	Anti-Hypoderma bovis and H. lineatum Hypodermins A mouse monoclonal antibody (mAb)
Expression platform	CHO
Isotypes	Mouse IgG
Bioactivity validation	Recombinant Hypoderma bovis and H. lineatum Hypodermins A antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Hypoderma bovis and H. lineatum antibodies in Hypoderma bovis and H. lineatum level test of animal Bovines/Cattle infectious disease with Hypodermiasis.
Product description	Anti-Hypoderma bovis and H. lineatum Hypodermins A mouse monoclonal antibody (mAb) is a mouse monoclonal antibody produced by CHO technology. The antibody is ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Hypoderma bovis and H. lineatum antibodies.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from sterile PBS, PH 7.4
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Cat No.	GMP-VT-P080-Tg001-Ab03,GMP-VT-P080-Tg001-Ab04
Pathogen	Hypoderma bovis and H. lineatum
Product Name	Anti-Hypoderma bovis and H. lineatum Hypodermins A human monoclonal antibody (mAb)
Expression platform	CHO
Isotypes	Human lgG1
Bioactivity validation	Recombinant Hypoderma bovis and H. lineatum Hypodermins A antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Hypoderma bovis and H. lineatum antibodies in Hypoderma bovis and H. lineatum level test of animal Bovines/Cattle infectious disease with Hypodermiasis.
Product description	Anti-Hypoderma bovis and H. lineatum Hypodermins A mouse monoclonal antibody (mAb) is a human monoclonal antibody produced by CHO. The antibody is ELISA validated as capture antibody and detection antibody pair.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from sterile PBS, PH 7.4
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Reference

Validation Data

Click to get more Data / Case study about the product.

Pathogen Information

Hypoderma bovis and H. lineatum are parasitic insects that belong to the family Oestridae, commonly known as cattle grubs or heel flies. These organisms have a unique life cycle in which they penetrate the host's skin by L3 stage larvae, which further develops through the subcutaneous tissue of the host. The infestation by these parasites, known as hypodermosis or warble fly infestation, is a major problem in livestock farming, particularly in tropical and subtropical regions.

The adult female Hypoderma bovis and H. lineatum flies deposit eggs on the hair shafts of cattle. After hatching, the larvae penetrate the skin and migrate through the subcutaneous tissue, where they can remain for up to a year before emerging from the host. The emergence of larvae from infected hosts creates an open wound that is susceptible to secondary bacterial infections and can adversely affect animal health and productivity.

Hyaluronidase, the primary protein involved in the pathogenicity of Hypoderma bovis and H. lineatum, is crucial for facilitating the penetration of host skin by larvae. This enzyme degrades hyaluronic acid, a component of the extracellular matrix, and aids in creating channels for the migration of L3 larvae through the host's tissue.

Cattle hosts with Hypoderma bovis and H. lineatum infestation exhibit several clinical symptoms, including restlessness, head shaking, and spinal discomfort. In severe cases, cattle may also show signs of weight loss, decreased milk production, and reduced fertility. The larvae of Hypoderma bovis and H. lineatum cause various changes in the skin and connective tissues of the host, which can lead to dermatitis, abscesses, or tissue necrosis.

Various diagnostic methods are available to detect Hypoderma bovis and H. lineatum infestations. Visual inspection of larvae and their migration paths under the skin is the most common method used in the field. However, molecular diagnostic methods such as PCR amplification of specific genes or proteins, including the hyaluronidase gene or protein, have proven to be more sensitive and specific for detecting these parasites. ELISA and serological testing for antibodies against Hypoderma antigens are additional diagnostic tools that can help detect infestation in cattle.

Control measures for Hypoderma bovis and H. lineatum infestations involve strategic use of chemical therapy, which targets the larvae at specific stages of development. Integrated pest management strategies that utilize biological control agents, pasture management, and good animal husbandry practices are also effective. Further research is necessary to develop novel control measures for managing Hypoderma bovis and H. lineatum infestations in cattle.

About GDU