Actinobacillus pleuropneumoniae serotype 5 antibody and antigen (recombinant protein)

Diagnostic anti-Actinobacillus pleuropneumoniae serotype 5 antibodies pairs and antigen for animal health (animal Swine/Porcine/Pig infectious disease swine pleuropneumonia) testing in ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA and POCT

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Product information

Catalog No. Description US $ Price (per mg)
GMP-VT-P124-Ag01 Recombinant Actinobacillus pleuropneumoniae serotype 5 protein $3090.00
GMP-VT-P124-Ab01 Anti-Actinobacillus pleuropneumoniae serotype 5 mouse monoclonal antibody (mAb) $3090.00
GMP-VT-P124-Ab02 Anti-Actinobacillus pleuropneumoniae serotype 5 mouse monoclonal antibody (mAb) $3090.00

Size: 1mg | 10mg | 100mg



Product Description

Cat No. GMP-VT-P124-Ag01
Product Name Recombinant Actinobacillus pleuropneumoniae serotype 5 protein
Pathogen Actinobacillus pleuropneumoniae serotype 5
Expression platform E.coli
Isotypes Recombinant Antigen
Bioactivity validation Anti-Actinobacillus pleuropneumoniae serotype 5 antibodies binding, Immunogen in Sandwich Elisa, lateral-flow tests, and other immunoassays as control material in Actinobacillus pleuropneumoniae serotype 5 level test of animal Swine/Porcine/Pig infectious disease with swine pleuropneumonia.
Tag His
Product description Recombinant Actinobacillus pleuropneumoniae serotype 5 proteinwas expressed in E.coli - based prokaryotic cell expression system and is expressed with 6 HIS tag at the C-terminus.
Purity Purity: ≥95% (SDS-PAGE)
Application Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation Lyophilized from sterile PBS, PH 7.4
Storage Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.


Cat No. GMP-VT-P124-Ab01,GMP-VT-P124-Ab02
Pathogen Actinobacillus pleuropneumoniae serotype 5
Product Name Anti-Actinobacillus pleuropneumoniae serotype 5 mouse monoclonal antibody (mAb)
Expression platform CHO
Isotypes Mouse IgG
Bioactivity validation Recombinant Actinobacillus pleuropneumoniae serotype 5 antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Actinobacillus pleuropneumoniae serotype 5 antibodies in Actinobacillus pleuropneumoniae serotype 5 level test of animal Swine/Porcine/Pig infectious disease with swine pleuropneumonia.
Product description Anti-Actinobacillus pleuropneumoniae serotype 5 mouse monoclonal antibody (mAb) is a mouse monoclonal antibody produced by CHO technology. The antibody is ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Actinobacillus pleuropneumoniae serotype 5 antibodies./td>
Purity Purity: ≥95% (SDS-PAGE)
Application Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation Lyophilized from sterile PBS, PH 7.4
Storage Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.


Reference




    Validation Data


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    Pathogen


    Actinobacillus pleuropneumoniae Serotype 5: Understanding a Swine Respiratory Pathogen

    Taxonomy and Description:

    Actinobacillus pleuropneumoniae serotype 5, a Gram-negative bacterium, is a member of the Pasteurellaceae family, which encompasses several pathogenic species known for their virulence in animals. This bacterium primarily targets swine, causing respiratory infections that pose significant economic challenges to the global pig farming industry. The specific serotype, 5, refers to the variation in the bacterial surface antigens, allowing for differentiation among various strains within the Actinobacillus pleuropneumoniae species.

    Pathogen Classification:

    In the realm of microbiology, Actinobacillus pleuropneumoniae serotype 5 falls under the category of prokaryotic organisms. Prokaryotes are unicellular organisms lacking membrane-bound nuclei and organelles, a characteristic feature distinguishing them from eukaryotic cells.

    Pathogen Structure:

    At the genetic level, Actinobacillus pleuropneumoniae serotype 5 harbors the apxIV gene, a significant element contributing to its pathogenicity. This gene encodes the RTX toxin, a member of the Repeats-in-Toxin (RTX) family. RTX toxins are known for their cytotoxic properties, disrupting host cell membranes and facilitating the bacterium's ability to evade the host immune system. The RTX toxin, specifically encoded by the apxIV gene, is a key virulence factor enabling the bacterium to cause disease in the porcine host.

    Hosts Infected and Diseases:

    Actinobacillus pleuropneumoniae serotype 5 displays a host specificity for pigs, particularly domesticated swine raised in intensive farming systems. When infection occurs, it leads to a severe respiratory disease known as Porcine Pleuropneumonia or Actinobacillosis. This disease primarily affects the lungs and pleura of pigs, causing acute pleuropneumonia. The symptoms of porcine pleuropneumonia can vary in severity, ranging from mild coughing and nasal discharge to severe respiratory distress, high fever, and, in fatal cases, sudden death. The economic impact of this disease is substantial, leading to significant losses in the swine industry due to decreased productivity, treatment costs, and increased mortality rates among infected animals.

    Diagnostic Methods:

    Diagnosing infections caused by Actinobacillus pleuropneumoniae serotype 5 involves a multifaceted approach, employing various diagnostic methods to ensure accuracy and reliability:

    Cultural Methods: Isolation and identification of the bacterium through cultural techniques remain a fundamental diagnostic approach. Clinical samples, such as lung tissues or pleural fluids, are cultured on specific agar media under controlled laboratory conditions. The characteristic colonies grown allow microbiologists to confirm the presence of Actinobacillus pleuropneumoniae serotype 5.

    Serological Tests: Serological assays, including enzyme-linked immunosorbent assay (ELISA) and agglutination tests, are employed to detect specific antibodies generated by the porcine host in response to Actinobacillus pleuropneumoniae serotype 5 infection. These tests enable indirect detection of the pathogen by identifying the host's immune response, providing valuable information for diagnosis.

    Molecular Methods (Nucleic Acids): Molecular techniques play a pivotal role in the precise and rapid detection of Actinobacillus pleuropneumoniae serotype 5 infections. Polymerase Chain Reaction (PCR), a widely utilized molecular tool, allows for the amplification of specific genetic markers, such as the apxIV gene. Targeting this gene in PCR assays enhances the sensitivity and specificity of the diagnostic process, enabling accurate identification of the pathogen within a short timeframe. Additionally, real-time PCR assays provide quantitative data, aiding in the determination of the bacterial load in clinical samples.

    Next-Generation Sequencing (NGS): With advancements in genomics, NGS technologies have revolutionized the field of microbial diagnostics. Whole-genome sequencing of Actinobacillus pleuropneumoniae serotype 5 isolates allows for in-depth genetic analysis, including the identification of virulence factors, antimicrobial resistance genes, and strain characterization. Comparative genomic analyses enable researchers to trace the evolutionary history of different strains, contributing valuable insights into the epidemiology and pathogenesis of Actinobacillus pleuropneumoniae infections.

    In conclusion, Actinobacillus pleuropneumoniae serotype 5 stands as a significant pathogen in the swine industry, causing substantial economic losses and animal welfare concerns. The integration of advanced diagnostic techniques, including molecular and genomic approaches, is crucial for timely and accurate detection of this bacterium, facilitating targeted control measures and mitigating the impact of porcine pleuropneumonia on pig populations worldwide. Ongoing research efforts continue to enhance our understanding of the bacterium's biology, virulence mechanisms, and host-pathogen interactions, paving the way for innovative strategies to combat this economically devastating disease in the future.



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