Infectious Haematopoietic Necrosis Virus antibody and antigen (recombinant protein)

Diagnostic anti-Infectious Haematopoietic Necrosis Virus antibodies pairs and antigen for animal health (animal Salmonid fish infectious disease infectious hematopoietic necrosis) testing in ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA and POCT

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Product information

Catalog No. Description US $ Price (per mg)
GMP-VT-P232-Ag01 Recombinant Infectious Haematopoietic Necrosis Virus protein $3090.00
GMP-VT-P232-Ab01 Anti-Infectious Haematopoietic Necrosis Virus mouse monoclonal antibody (mAb) $3090.00
GMP-VT-P232-Ab02 Anti-Infectious Haematopoietic Necrosis Virus mouse monoclonal antibody (mAb) $3090.00

Size: 1mg | 10mg | 100mg



Product Description

Cat No. GMP-VT-P232-Ag01
Product Name Recombinant Infectious Haematopoietic Necrosis Virus protein
Pathogen Infectious Haematopoietic Necrosis Virus
Expression platform E.coli
Isotypes Recombinant Antigen
Bioactivity validation Anti-Infectious Haematopoietic Necrosis Virus antibodies binding, Immunogen in Sandwich Elisa, lateral-flow tests, and other immunoassays as control material in Infectious Haematopoietic Necrosis Virus level test of animal Salmonid fish infectious disease with infectious hematopoietic necrosis.
Tag His
Product description Recombinant Infectious Haematopoietic Necrosis Virus proteinwas expressed in E.coli - based prokaryotic cell expression system and is expressed with 6 HIS tag at the C-terminus.
Purity Purity: ≥95% (SDS-PAGE)
Application Paired antibody immunoassay validation in Sandwich ELISA, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation Lyophilized from GM's Protein Stability Buffer2 (PSB2,Confidential Ingredients) or PBS (pH7.4);
For PSB2, reconstituted with 0.9% sodium chloride;
For PBS, reconstituted with ddH2O.
Storage Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.


Cat No. GMP-VT-P232-Ab01,GMP-VT-P232-Ab02
Pathogen Infectious Haematopoietic Necrosis Virus
Product Name Anti-Infectious Haematopoietic Necrosis Virus mouse monoclonal antibody (mAb)
Expression platform CHO
Isotypes Mouse IgG
Bioactivity validation Recombinant Infectious Haematopoietic Necrosis Virus antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Infectious Haematopoietic Necrosis Virus antibodies in Infectious Haematopoietic Necrosis Virus level test of animal Salmonid fish infectious disease with infectious hematopoietic necrosis.
Product description Anti-Infectious Haematopoietic Necrosis Virus mouse monoclonal antibody (mAb) is a mouse monoclonal antibody produced by CHO technology. The antibody is ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Infectious Haematopoietic Necrosis Virus antibodies./td>
Purity Purity: ≥95% (SDS-PAGE)
Application Paired antibody immunoassay validation in Sandwich ELISA, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation Lyophilized from GM's Protein Stability Buffer2 (PSB2,Confidential Ingredients) or PBS (pH7.4);
For PSB2, reconstituted with 0.9% sodium chloride;
For PBS, reconstituted with ddH2O.
Storage Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.


Reference




    Validation Data


    Click to get more Data / Case study about the product.



    Pathogen


    IHNV is a viral pathogen that primarily infects salmonid fish, including rainbow trout, Atlantic salmon, and Pacific salmon. The virus was first identified in the United States in the 1950s and has since been found in various regions worldwide. IHNV belongs to the family Rhabdoviridae, which includes several other fish viruses and the rabies virus.

    The genome of IHNV is composed of negative-sense single-stranded RNA and contains five genes: nucleoprotein (N), phosphoprotein (P), matrix protein (M), glycoprotein (G), and RNA-dependent RNA polymerase (L). N protein encapsulates viral RNA, P protein interacts with N protein to form ribonucleoprotein complexes, M protein plays an important role in virion assembly, and G protein facilitates viral entry by binding to host cells. The L protein serves as an RNA-dependent RNA polymerase that allows the virus to replicate its RNA genome.

    IHNV causes a severe disease known as infectious hematopoietic necrosis (IHN), which affects the internal organs and tissues of infected fish. The disease is characterized by anemia, petechial hemorrhages, and necrosis. Infected fish may exhibit external clinical signs such as darkened skin, distended abdomen, and exophthalmia. These symptoms are a result of the damage caused to the hematopoietic system and other organs.

    The pathogenesis of IHN involves viral replication in susceptible fish tissues, leading to the induction of the host immune response. The immune response can cause the destruction of infected cells through apoptosis, leading to tissue damage and the release of inflammatory cytokines. In addition, the virus can infect hematopoietic stem cells, leading to a decline in the number of circulating blood cells and anemia.

    Several diagnostic methods have been developed to identify IHNV infections in salmonid fish. These methods include PCR (polymerase chain reaction) and ELISA (enzyme-linked immunosorbent assay), which detect viral RNA or antibodies. These diagnostic methods target the N and G genes of IHNV. In addition, virus isolation and histopathological examination can also be utilized to diagnose IHN infections. Virus isolation involves the cultivation of viral particles on cell culture, while histopathological examination involves the microscopic analysis of tissue sections to identify characteristic lesions.

    The control of IHN relies on the implementation of various measures, such as fish vaccination, biosecurity, and quarantine protocols. Vaccination is the most effective control measure, as it can stimulate the production of specific antibodies that can neutralize the virus. Biosecurity measures, such as preventing the introduction of infected fish or equipment, can also prevent the spread of the virus between farms. Quarantine protocols involve isolating new fish arrivals to monitor them for signs of infection before introducing them into the main population.

    In conclusion, IHNV is a highly pathogenic virus that infects salmonid fish, causing severe disease known as infectious hematopoietic necrosis (IHN). The virus primarily targets the hematopoietic system and other organs, leading to anemia, petechial hemorrhages, and necrosis. Several diagnostic methods have been developed to identify IHNV infections, including PCR, ELISA, virus isolation, and histopathological examination. The control of IHN relies on the implementation of various measures, such as fish vaccination, biosecurity, and quarantine protocols.



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