Escherichia coli K99 antibody and antigen (recombinant protein)

Diagnostic anti-Escherichia coli K99 antibodies pairs and antigen for animal health (animal Bovines/Cattle infectious disease diarrhea) testing in ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA and POCT

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Product information

Catalog No. Description US $ Price (per mg)
GMP-VT-P288-Ag01 Recombinant Escherichia coli K99 protein $3090.00
GMP-VT-P288-Ab01 Anti-Escherichia coli K99 mouse monoclonal antibody (mAb) $3090.00
GMP-VT-P288-Ab02 Anti-Escherichia coli K99 mouse monoclonal antibody (mAb) $3090.00

Size: 1mg | 10mg | 100mg



Product Description

Cat No. GMP-VT-P288-Ag01
Product Name Recombinant Escherichia coli K99 protein
Pathogen Escherichia coli K99
Expression platform E.coli
Isotypes Recombinant Antigen
Bioactivity validation Anti-Escherichia coli K99 antibodies binding, Immunogen in Sandwich Elisa, lateral-flow tests, and other immunoassays as control material in Escherichia coli K99 level test of animal Bovines/Cattle infectious disease with diarrhea.
Tag His
Product description Recombinant Escherichia coli K99 proteinwas expressed in E.coli - based prokaryotic cell expression system and is expressed with 6 HIS tag at the C-terminus.
Purity Purity: ≥95% (SDS-PAGE)
Application Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation Lyophilized from sterile PBS, PH 7.4
Storage Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.


Cat No. GMP-VT-P288-Ab01,GMP-VT-P288-Ab02
Pathogen Escherichia coli K99
Product Name Anti-Escherichia coli K99 mouse monoclonal antibody (mAb)
Expression platform CHO
Isotypes Mouse IgG
Bioactivity validation Recombinant Escherichia coli K99 antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Escherichia coli K99 antibodies in Escherichia coli K99 level test of animal Bovines/Cattle infectious disease with diarrhea.
Product description Anti-Escherichia coli K99 mouse monoclonal antibody (mAb) is a mouse monoclonal antibody produced by CHO technology. The antibody is ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Escherichia coli K99 antibodies./td>
Purity Purity: ≥95% (SDS-PAGE)
Application Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation Lyophilized from sterile PBS, PH 7.4
Storage Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.


Reference




    Validation Data


    Click to get more Data / Case study about the product.



    Pathogen


    Bovine Rotavirus: A Comprehensive Overview

    Pathogen Name and Description:

    Bovine Rotavirus, a pathogen with considerable implications for the cattle industry, is a double-stranded RNA virus belonging to the Reoviridae family. This viral agent primarily targets cattle, particularly affecting young calves. The infection initiated by Bovine Rotavirus leads to severe gastrointestinal issues and can result in debilitating diarrhea in affected calves, significantly impacting the economic landscape of the cattle industry.

    Pathogen Classification:

    Bovine Rotavirus is classified within the realm of viruses, standing as a distinguished member of the Reoviridae family. It is important to note that this pathogen does not fall within the domains of eukaryotic or prokaryotic organisms.

    Pathogen Structure (Main Gene and Protein):

    The structural composition of Bovine Rotavirus is notably intricate, characterized by multiple protein layers. These structural proteins include VP1, VP2, VP3, VP4, VP6, and VP7, all of which play indispensable roles in the overall structure and function of the virus.

    VP1 and VP2: These proteins are essential components of the core structure, preserving the viral RNA genome.

    VP3 and VP6: These proteins participate in the formation of the intermediate layer, contributing to the stability of the viral particle.

    VP4 and VP7: These proteins are situated on the outer capsid of the virus and are crucial for attachment to host cells, enabling the virus to initiate the infection process.

    Hosts Infected and Associated Diseases:

    Hosts: Bovine Rotavirus is a highly specialized pathogen that primarily infects cattle, with a particular affinity for infecting young calves. While other animals are not typically affected, the primary concern revolves around the impact on the cattle population.

    Associated Diseases: The infection caused by Bovine Rotavirus often results in a distressing and sometimes fatal condition known as calf diarrhea. Calves afflicted by this ailment can exhibit severe dehydration and suffer from nutritional deficiencies, significantly impacting their health. Beyond the immediate welfare of the animals, the economic consequences for the cattle industry are substantial, with losses incurred through reduced productivity and increased healthcare costs.

    Diagnostic Methods:

    Detecting Bovine Rotavirus is pivotal in managing its impact on cattle. Various diagnostic methods are employed to identify the presence of the virus, with a particular focus on nucleic acid-based techniques:

    Polymerase Chain Reaction (PCR): PCR is a highly sensitive and specific method for the detection of Bovine Rotavirus. The technique targets specific genes or genomic regions unique to the virus, with the VP6 gene and NSP3 gene being common targets in diagnostic assays. The VP6 gene, in particular, is favored for PCR-based detection due to its conserved nature.

    Serotyping with VP7 and VP4: The VP7 and VP4 proteins play a critical role in virus classification, enabling serotyping and contributing to a better understanding of the virus's subtypes.

    In addition to nucleic acid-based methods, enzyme-linked immunosorbent assay (ELISA) methods are employed to detect viral antigens. ELISA assays are useful in screening for the presence of viral particles, including VP6, within fecal samples collected from infected cattle.

    Conclusion:

    Bovine Rotavirus is a significant concern in the cattle industry, given its ability to cause severe gastrointestinal distress and calf diarrhea, leading to substantial economic repercussions. Understanding the virus's structure, the hosts it infects, the associated diseases, and diagnostic methods is pivotal in the management and prevention of its impact on cattle populations.

    In summary, Bovine Rotavirus, while highly specialized in its host range, poses a notable threat to calf health and the livestock industry at large. Comprehensive knowledge and effective diagnostic tools are critical components in mitigating its impact on cattle and, by extension, the agricultural economy.



    About GDU


    GDU

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