Diagnostic anti-Bovine leukemia virus (BLV) antibodies pairs and antigens for animal health (animal infectious disease ) testing in ELISA test,competitive ELISA test, blocking ELISA test, Lateral flow immunoassay (LFIA), colloidal gold immunochromatographic assay, Chemiluminescent immunoassay (CLIA), turbidimetric inhibition immuno assay (TINIA), and immunonephelometry

Definition of the disease: Bovine leukemia virus (BLV), the etiological agent of enzootic bovine leukosis (EBL), is a C-type retrovirus of cattle with a worldwide distribution (5). The virus induces a persistent lymphocytosis and in some cases lymphoid tumors in domestic cattle. The BLV envelope (Env) glycoprotein, which consists of the gp51 outer membrane glycoprotein and the gp30 transmembrane glycoprotein, is directly involved in infectivity events and, like the p24 major structural protein, can elicit a strong immune response in infected cattle (23). Serological diagnosis of EBL is mainly based on screening of field samples for gp51 antibodies. It follows that diagnostic procedures that use native gp51 constitute a prerequisite for the design of an efficient EBL eradication program. In recent decades different serological methods, such as the agar gel immunodiffusion test (AGID) and the enzyme-linked immunosorbent assay (ELISA), have been developed. ELISAs are based on the use of partially purified BLV gp51 and monoclonal antibodies (MAbs) against BLV gp51 epitopes (4, 17). These procedures are particularly useful for samples with low antibody titers, such as milk samples or pooled sera. A permanent fetal lamb kidney (FLK) cell line chronically infected with BLV (FLK-BLV) is used to produce gp51. Although highly productive BLV-infected cell lines are available, their production is rather laborious, expensive, and time-consuming.

Genemedi produces core animal health diagnostic ingredients-validated antibodies pairs Mouse anti-Bovine leukemia virus (BLV) monoclonal antibody and Bovine leukemia virus (BLV) antigens for rapid test kit of animal infectious disease with Bovine leukemia virus (BLV) to evaluate the animal health of Ruminants.

The paired antibodies are both monoclonal antibody(mab).

All the antibodies and antiges of animal disease test are suitable for in functional ELISA, and other immunoassays in dignostics.The antibody can act as a capture antibody and detection antibody. Antigens are validated as positive control materials.

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GMP-AD-Ruminants-13Ab, GMP-AD-Ruminants-13Ag