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25 hydroxyvitamin D (25-OH-(VD3+VD2)) antibody and antigen (hapten, BSA/OVA conjugated)
Diagnostic anti-25 hydroxyvitamin D (25-OH-(VD3+VD2)) antibodies (anti-25 hydroxyvitamin D, anti-25-OH-(VD3+VD2)) and diagnostic hapten-carrier conjugates BSA-25 hydroxyvitamin D (25-OH-(VD3+VD2)), OVA-25 hydroxyvitamin D (25-OH-(VD3+VD2)), KLH-25 hydroxyvitamin D (25-OH-(VD3+VD2)) antigens for Metabolic diseases 25-OH-(VD3+VD2) detection in ELISA test, competitive ELISA, Lateral flow immunoassay (LFIA), CLIA, TINIA and POCT.
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Product information
| Catalog No. | Description | US $ Price (per mg) |
|---|---|---|
| GMP-SMT-25-OH-(VD3+VD2)-Ag01 | BSA-25 hydroxyvitamin D (25-OH-(VD3+VD2)) | $756.00 |
| GMP-SMT-25-OH-(VD3+VD2)-Ag02 | OVA-25 hydroxyvitamin D (25-OH-(VD3+VD2)) | $756.00 |
| GMP-SMT-25-OH-(VD3+VD2)-Ab01 | Anti-human 25 hydroxyvitamin D (25-OH-(VD3+VD2)) rabbit monoclonal antibody (rab mAb) | $1953.00 |
| GMP-SMT-25-OH-(VD3+VD2)-Ab02 | Anti-human 25 hydroxyvitamin D (25-OH-(VD3+VD2)) rabbit monoclonal antibody (rab mAb) | $1953.00 |
Size： 1mg | 10mg | 100mg
Product Description
BSA-25 hydroxyvitamin D (25-OH-(VD3+VD2))
| Cat No. | GMP-SMT-25-OH-(VD3+VD2)-Ag01 |
| Product Name | BSA-25 hydroxyvitamin D (25-OH-(VD3+VD2)) |
| Target | 25 hydroxyvitamin D (25-OH-(VD3+VD2)) |
| Expression platform | Synthetic |
| Bioactivity validation | 25 hydroxyvitamin D (25-OH-(VD3+VD2)) antibodies binding, Immunogen in Sandwich Elisa, lateral-flow tests, and other immunoassays as control material in 25-OH-(VD3+VD2) level test of Metabolic diseases (bone fracture, coronary heart disease, cancer, and all-cause mortality) and related syndrome evaluation |
| Products description | Competitive immunoassay-validated hapten-carrier conjugates BSA-25 hydroxyvitamin D (25-OH-(VD3+VD2)) / OVA-25 hydroxyvitamin D (25-OH-(VD3+VD2)) is Synthetic. |
| Purity | High (TBD) |
| Application | Competitive immunoassay validation (Competitive ELISA), ELISA, Lateral flow immunoassay (LFIA),colloidal gold immunochromatographic assay, Chemiluminescent immunoassay (CLIA),turbidimetric inhibition immuno assay (TINIA) and Immunonephelometry. |
| Formulation | Lyophilized from sterile PBS, PH 7.4 |
| Storage | Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles. |
OVA-25 hydroxyvitamin D (25-OH-(VD3+VD2))
| Cat No. | GMP-SMT-25-OH-(VD3+VD2)-Ag02 |
| Product Name | OVA-25 hydroxyvitamin D (25-OH-(VD3+VD2)) |
| Target | 25 hydroxyvitamin D (25-OH-(VD3+VD2)) |
| Expression platform | Synthetic |
| Bioactivity validation | 25 hydroxyvitamin D (25-OH-(VD3+VD2)) antibodies binding, Immunogen in Sandwich Elisa, lateral-flow tests, and other immunoassays as control material in 25-OH-(VD3+VD2) level test of Metabolic diseases (bone fracture, coronary heart disease, cancer, and all-cause mortality) and related syndrome evaluation/td> |
| Products description | Competitive immunoassay-validated hapten-carrier conjugates BSA-25 hydroxyvitamin D (25-OH-(VD3+VD2)) / OVA-25 hydroxyvitamin D (25-OH-(VD3+VD2)) is Synthetic. |
| Purity | High (TBD) |
| Application | Competitive immunoassay validation (Competitive ELISA), ELISA, Lateral flow immunoassay (LFIA),colloidal gold immunochromatographic assay, Chemiluminescent immunoassay (CLIA),turbidimetric inhibition immuno assay (TINIA) and Immunonephelometry. |
| Formulation | Lyophilized from sterile PBS, PH 7.4 |
| Storage | Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles. |
Anti-human 25 hydroxyvitamin D (25-OH-(VD3+VD2)) rabbit monoclonal antibody (rab mAb)
| Cat No. | GMP-SMT-25-OH-(VD3+VD2)-Ab01 |
| Product Name | Anti-human 25 hydroxyvitamin D (25-OH-(VD3+VD2)) rabbit monoclonal antibody (rab mAb) |
| Target | 25 hydroxyvitamin D (25-OH-(VD3+VD2)) |
| Expression platform | CHO |
| Isotypes | Rabbit IgG |
| Bioactivity validation | 25 hydroxyvitamin D (25-OH-(VD3+VD2)) antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other 25 hydroxyvitamin D (25-OH-(VD3+VD2)) antibodies in 25-OH-(VD3+VD2) level test of Metabolic diseases (bone fracture, coronary heart disease, cancer, and all-cause mortality) and related syndrome evaluation. |
| Tag | rabbit Fc |
| Products description | Anti-human 25 hydroxyvitamin D (25-OH-(VD3+VD2)) rabbit monoclonal antibody (rab mAb) is a rabbit monoclonal antibody produced by CHO technology. |
| Purity | High (TBD) |
| Application | Competitive immunoassay validation (Competitive ELISA), ELISA, Lateral flow immunoassay (LFIA),colloidal gold immunochromatographic assay, Chemiluminescent immunoassay (CLIA),turbidimetric inhibition immuno assay (TINIA) and Immunonephelometry. |
| Formulation | Lyophilized from sterile PBS, PH 7.4 |
| Storage | Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles. |
Anti-human 25 hydroxyvitamin D (25-OH-(VD3+VD2)) rabbit monoclonal antibody (rab mAb)
| Cat No. | GMP-SMT-25-OH-(VD3+VD2)-Ab02 |
| Product Name | Anti-human 25 hydroxyvitamin D (25-OH-(VD3+VD2)) rabbit monoclonal antibody (rab mAb) |
| Target | 25 hydroxyvitamin D (25-OH-(VD3+VD2)) |
| Expression platform | CHO |
| Isotypes | Rabbit IgG1 |
| Bioactivity validation | 25 hydroxyvitamin D (25-OH-(VD3+VD2)) antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other 25 hydroxyvitamin D (25-OH-(VD3+VD2)) antibodies in 25-OH-(VD3+VD2) level test of Metabolic diseases (bone fracture, coronary heart disease, cancer, and all-cause mortality) and related syndrome evaluation. |
| Tag | rabbit Fc |
| Products description | Anti-human 25 hydroxyvitamin D (25-OH-(VD3+VD2)) rabbit monoclonal antibody (rab mAb) is a rabbit monoclonal antibody produced by CHO. |
| Purity | Purity: ≥95% （SDS-PAGE） |
| Application | Competitive immunoassay validation (Competitive ELISA), ELISA, Lateral flow immunoassay (LFIA), colloidal gold immunochromatographic assay, Chemiluminescent immunoassay (CLIA),turbidimetric inhibition immuno assay (TINIA) and Immunonephelometry. |
| Formulation | Supplied as a 0.2 μM filtered solution of PBS, PH7.4. |
| Storage | Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles. |
Reference
Validation Data
Direct chemiluminescence platform
Total 25-OH-VD identification ability
Genemedi's 25-OH-VD sandwich antibodies (GMP-SMT-25-OH-VD3/2-Ab01 and GMP-SMT-25-OH-VD3/2-Ab02) were validated on the direct chemiluminescence platform. The total amount of 25-OH-VD in the sample may be successfully detected thanks to the good cross-reactivity of 25-OH-VD2 and 25-OH-VD3.
Figure 1. Total 25-OH-VD recognition ability
Sample coincidence rate
The VD sandwich antibody made by Genemedi was validated using a direct
chemiluminescence platform with the industry's gold standard LC-MS/MS as a
control to increase the accuracy of the 25-OH-VD immunoassay.
When compared to the mass spectrometry detection results, these
antibodies exhibited excellent consistency, with an R2 of up to 0.9718
and a customer-verified R2 of 0.963. The sandwich approach greatly
improved immunodetection accuracy.
Figure 2. Self-assessment clinical comparative analysis
Figure 3. Clinical comparative analysis of customer testing
Enzymatic chemiluminescence platform
standard curve line
The detection concentration range for Genemedi's 25-OH-VD sandwich antibodies (GMP-SMT-25-OH-VD3/2-Ab01 and GMP-SMT-25-OH-VD3/2-Ab02) is 0.1 to 80ng/mL.
Figure 4. Standard curve
Sample compliance rate
Detection employing 25-OH-VD sandwich antibodies (GMP-SMT-25-OH-VD3/2-Ab01 and GMP-SMT-25-OH-VD3/2-Ab02) validated by an enzymatic chemiluminescence platform using an R2 of 0.9741, the data demonstrated strong consistency using mass spectrometry (LC-MS/MS).
Figure 5. Clinical comparative analysis
Click to get more Data / Case study about the product.
Target/Biomarker information
Vitamin D is a nutrient that is essential for healthy bones and teeth. There are two forms of vitamin D that are important for nutrition: vitamin D2 and vitamin D3. Vitamin D2 mainly comes from fortified foods like breakfast cereals, milk, and other dairy items. Vitamin D3 is made by your own body when you are exposed to sunlight. It is also found in some foods, including eggs and fatty fish, such as salmon, tuna, and mackerel. In your bloodstream, vitamin D2 and vitamin D3 are changed into a form of vitamin D called 25 hydroxyvitamin D, also known as 25(OH)D. A vitamin D blood test measures the level of 25(OH)D in your blood. Abnormal levels of vitamin D can indicate bone disorders, nutrition problems, organ damage, or other medical conditions. A vitamin D test is used to screen for or monitor bone disorders. It is also sometimes used to check vitamin D levels in people with chronic illnesses such as asthma, psoriasis, and certain autoimmune diseases.
About GDU
GDU helps global diagnostic partners in high quality of raw material discovery, development, and application. GDU believes in Protein&antibody Innovation for more reliable diagnostic solutions.
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