Leptospira antibody and antigen (recombinant protein)

Diagnostic anti-Leptospira antibodies pairs and antigen for animal health (animal Dog/Canine, Swine/Porcine/Pig infectious disease Leptospirosis) testing in ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA and POCT

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Product information

Catalog No. Description US $ Price (per mg)
GMP-VT-P026-Ag01 Recombinant Leptospira protein $3090.00
GMP-VT-P026-Ab01 Anti-Leptospira mouse monoclonal antibody (mAb) $3090.00
GMP-VT-P026-Ab02 Anti-Leptospira mouse monoclonal antibody (mAb) $3090.00

Size: 1mg | 10mg | 100mg



Product Description

Cat No. GMP-VT-P026-Ag01
Product Name Recombinant Leptospira protein
Pathogen Leptospira
Expression platform E.coli
Isotypes Recombinant Antigen
Bioactivity validation Anti-Leptospira antibodies binding, Immunogen in Sandwich Elisa, lateral-flow tests, and other immunoassays as control material in Leptospira level test of animal Dog/Canine, Swine/Porcine/Pig infectious disease with Leptospirosis.
Tag His
Product description Recombinant Leptospira proteinwas expressed in E.coli - based prokaryotic cell expression system and is expressed with 6 HIS tag at the C-terminus.
Purity Purity: ≥95% (SDS-PAGE)
Application Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation Lyophilized from sterile PBS, PH 7.4
Storage Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.


Cat No. GMP-VT-P026-Ab01,GMP-VT-P026-Ab02
Pathogen Leptospira
Product Name Anti-Leptospira mouse monoclonal antibody (mAb)
Expression platform CHO
Isotypes Mouse IgG
Bioactivity validation Recombinant Leptospira antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Leptospira antibodies in Leptospira level test of animal Dog/Canine, Swine/Porcine/Pig infectious disease with Leptospirosis.
Product description Anti-Leptospira mouse monoclonal antibody (mAb) is a mouse monoclonal antibody produced by CHO technology. The antibody is ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Leptospira antibodies./td>
Purity Purity: ≥95% (SDS-PAGE)
Application Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation Lyophilized from sterile PBS, PH 7.4
Storage Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.


Reference




    Validation Data


    Click to get more Data / Case study about the product.



    Pathogen


    Leptospira is a genus of spirochete bacteria known for its helical shape and remarkable motility. The genus includes three named species: Leptospira interrogans, Leptospira biflexa, and Leptospira kirschneri. Leptospira is a gram-negative bacterium that belongs to the family Leptospiraceae and order Spirochaetales. The genus is mainly characterized by its twisted spiral shape, periplasmic flagella, and unique ability to swim and penetrate different host tissues.

    The genome of Leptospira consists of several genes encoding for proteins involved in motility, adhesion, and immune evasion. These proteins are highly conserved and critical for the pathogenesis of leptospirosis, the infectious disease caused by Leptospira. Some of the crucial genes include flaB, which encodes for flagellar protein, lipL41, which encodes for outer membrane protein, as well as ligB and ligA, which encode for immunoglobulin-like surface-exposed proteins. Additionally, Leptospira's major outer membrane proteins are LipL32 and LipL41, which are crucial for bacterial survival and virulence.

    Leptospira has a wide host range and can infect a vast array of mammals, including rodents, cattle, pigs, horses, dogs, and humans. As a result, the diseases caused by Leptospira in humans are collectively termed leptospirosis. Leptospirosis is a globally distributed zoonotic disease that occurs primarily in tropical regions. The symptoms of leptospirosis in humans can range from mild flu-like symptoms to severe life-threatening conditions such as Weil's disease and pulmonary hemorrhage syndrome. The severity of the disease can depend on the virulence of the Leptospira strain, host susceptibility, and environmental factors.

    To diagnose leptospirosis, various methods are employed such as serological tests and nucleic acid amplification techniques (NAATs). Serological tests detect antibodies produced by the host in response to Leptospira infection, while NAATs detect the presence of Leptospira DNA in blood or urine samples. The commonly targeted genes by NAATs include lipL32, lipL41, secY, and 16S rRNA.

    Leptospira's virulence mechanisms are multifactorial and complex. One of these mechanisms is its ability to evade the host immune response by expressing various immunoglobulin-like proteins. These proteins bind to the host extracellular matrix proteins, thereby avoiding detection by the host's immune system. Leptospira also has the ability to modulate host cell signaling pathways, leading to changes in gene expression, cytokine release, and apoptosis. Additionally, Leptospira's motility and chemotaxis allow it to efficiently penetrate the host's tissues, leading to dissemination and systemic infection.

    In conclusion, Leptospira is a pathogenic spirochete bacterium that causes leptospirosis, a globally distributed zoonotic disease with a broad clinical spectrum. The genus's virulence mechanisms, genetic makeup, host range, and epidemiology make it a challenging pathogen to study, diagnose, and treat. However, ongoing research into the genetic and molecular basis of Leptospira pathogenesis has facilitated the development of novel diagnostic tools and potential therapeutic targets.



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