Canine leptospira antibody and antigen (recombinant protein)

Diagnostic anti-Canine leptospira antibodies pairs and antigen for animal health (animal Cat/Feline, Dog/Canine infectious disease Leptospirosis) testing in ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA and POCT

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Product information

Catalog No. Description US $ Price (per mg)
GMP-VT-P028-Ag01 Recombinant Canine leptospira protein $3090.00
GMP-VT-P028-Ab01 Anti-Canine leptospira mouse monoclonal antibody (mAb) $3090.00
GMP-VT-P028-Ab02 Anti-Canine leptospira mouse monoclonal antibody (mAb) $3090.00

Size: 1mg | 10mg | 100mg



Product Description

Cat No. GMP-VT-P028-Ag01
Product Name Recombinant Canine leptospira protein
Pathogen Canine leptospira
Expression platform E.coli
Isotypes Recombinant Antigen
Bioactivity validation Anti-Canine leptospira antibodies binding, Immunogen in Sandwich Elisa, lateral-flow tests, and other immunoassays as control material in Canine leptospira level test of animal Cat/Feline, Dog/Canine infectious disease with Leptospirosis.
Tag His
Product description Recombinant Canine leptospira proteinwas expressed in E.coli - based prokaryotic cell expression system and is expressed with 6 HIS tag at the C-terminus.
Purity Purity: ≥95% (SDS-PAGE)
Application Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation Lyophilized from sterile PBS, PH 7.4
Storage Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.


Cat No. GMP-VT-P028-Ab01,GMP-VT-P028-Ab02
Pathogen Canine leptospira
Product Name Anti-Canine leptospira mouse monoclonal antibody (mAb)
Expression platform CHO
Isotypes Mouse IgG
Bioactivity validation Recombinant Canine leptospira antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Canine leptospira antibodies in Canine leptospira level test of animal Cat/Feline, Dog/Canine infectious disease with Leptospirosis.
Product description Anti-Canine leptospira mouse monoclonal antibody (mAb) is a mouse monoclonal antibody produced by CHO technology. The antibody is ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Canine leptospira antibodies./td>
Purity Purity: ≥95% (SDS-PAGE)
Application Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation Lyophilized from sterile PBS, PH 7.4
Storage Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.


Reference




    Validation Data


    Click to get more Data / Case study about the product.



    Pathogen


    Canine Leptospira, scientifically known as Leptospira interrogans, is a pathogenic bacterium that belongs to the phylum Spirochaetes and the family Leptospiraceae. It is a Gram-negative, spirochete-shaped bacterium with periplasmic flagella, which provide it with high motility. Canine Leptospira is further classified into serovars, with two notable serovars being Canicola and Icterohaemorrhagiae.

    The main gene and protein associated with the virulence and pathogenicity of Canine Leptospira are LipL32, which encodes an outer membrane lipoprotein. LipL32 is a key protein involved in host-pathogen interactions and immune responses, playing a crucial role in the infectivity of the bacterium.

    Canine Leptospira primarily infects dogs but can also affect other mammalian hosts, including humans. In dogs, it causes a spectrum of diseases collectively known as canine leptospirosis. This zoonotic disease is characterized by systemic manifestations and can present with various clinical signs. Dogs infected with Canine Leptospira may exhibit symptoms such as fever, myalgia (muscle pain), vomiting, diarrhea, jaundice, kidney damage, and potentially fatal complications. Severe cases can result in hemorrhage, organ failure, and death.

    Transmission of Canine Leptospira occurs through contact with infected animals or their urine-contaminated environments. Dogs can become infected by direct contact with urine, ingestion of contaminated water or food, or through broken skin or mucous membranes. The bacteria can survive in moist environments such as soil and water for extended periods, increasing the risk of exposure. Additionally, infected dogs can shed the bacteria in their urine for months, further contributing to the spread of the disease.

    To diagnose Canine Leptospira infections, various diagnostic methods are available. The gold standard serological test is the Microscopic Agglutination Test (MAT). This test detects specific antibodies against Leptospira in the serum and aids in identifying the infecting serovar. Polymerase Chain Reaction (PCR) techniques are also widely used for the detection of Canine Leptospira. PCR-based assays target specific genes, including LipL32 and the conserved 16S ribosomal RNA gene (rrs or 16S rRNA), providing highly sensitive and specific results. The detection of Canine Leptospira DNA confirms the presence of the pathogen.

    Prevention and control of Canine Leptospira infections involve several measures. Vaccination against the common serovars is available for dogs, which can help reduce the risk and severity of the disease. Maintaining good hygiene practices, such as avoiding contact with potentially contaminated environments and ensuring proper disposal of infected animal waste, is essential. Regular monitoring of water sources and control of rodents, which can act as reservoir hosts for the bacterium, also contribute to prevention efforts.

    In summary, Canine Leptospira is a pathogenic bacterium that primarily affects dogs but can also cause zoonotic infections in humans. It is a Gram-negative spirochete with motility provided by periplasmic flagella. Canine leptospirosis presents with systemic manifestations and can lead to severe complications in infected dogs. Diagnosis involves serological tests like MAT and molecular techniques such as PCR. Vaccination, hygiene practices, and rodent control are important for prevention. Understanding the biology and transmission dynamics of Canine Leptospira is crucial in safeguarding the health of both animals and humans.



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