Besnoitia Besnoiti antibody and antigen (recombinant protein)

Diagnostic anti-Besnoitia Besnoiti antibodies pairs and antigen for animal health (animal Bovines/Cattle infectious disease Bovine besnoitiosis) testing in ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA and POCT

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Product information

Catalog No. Description US $ Price (per mg)
GMP-VT-P051-Ag01 Recombinant Besnoitia Besnoiti protein $3090.00
GMP-VT-P051-Ab01 Anti-Besnoitia Besnoiti mouse monoclonal antibody (mAb) $3090.00
GMP-VT-P051-Ab02 Anti-Besnoitia Besnoiti mouse monoclonal antibody (mAb) $3090.00

Size: 1mg | 10mg | 100mg



Product Description

Cat No. GMP-VT-P051-Ag01
Product Name Recombinant Besnoitia Besnoiti protein
Pathogen Besnoitia Besnoiti
Expression platform E.coli
Isotypes Recombinant Antigen
Bioactivity validation Anti-Besnoitia Besnoiti antibodies binding, Immunogen in Sandwich Elisa, lateral-flow tests, and other immunoassays as control material in Besnoitia Besnoiti level test of animal Bovines/Cattle infectious disease with Bovine besnoitiosis.
Tag His
Product description Recombinant Besnoitia Besnoiti proteinwas expressed in E.coli - based prokaryotic cell expression system and is expressed with 6 HIS tag at the C-terminus.
Purity Purity: ≥95% (SDS-PAGE)
Application Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation Lyophilized from sterile PBS, PH 7.4
Storage Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.


Cat No. GMP-VT-P051-Ab01,GMP-VT-P051-Ab02
Pathogen Besnoitia Besnoiti
Product Name Anti-Besnoitia Besnoiti mouse monoclonal antibody (mAb)
Expression platform CHO
Isotypes Mouse IgG
Bioactivity validation Recombinant Besnoitia Besnoiti antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Besnoitia Besnoiti antibodies in Besnoitia Besnoiti level test of animal Bovines/Cattle infectious disease with Bovine besnoitiosis.
Product description Anti-Besnoitia Besnoiti mouse monoclonal antibody (mAb) is a mouse monoclonal antibody produced by CHO technology. The antibody is ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Besnoitia Besnoiti antibodies./td>
Purity Purity: ≥95% (SDS-PAGE)
Application Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation Lyophilized from sterile PBS, PH 7.4
Storage Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.


Reference




    Validation Data


    Click to get more Data / Case study about the product.



    Pathogen


    Besnoitia besnoiti, also known as bovine besnoitiosis or simply Besnoitia, is a protozoan parasite that belongs to the phylum Apicomplexa. Within this phylum, it falls under the class Conoidasida, order Eucoccidiorida, family Sarcocystidae, and genus Besnoitia. This obligate intracellular parasite has a complex cellular structure, which is characteristic of apicomplexan parasites.

    The life cycle of Besnoitia besnoiti involves both sexual and asexual stages. The parasite enters the host through the ingestion of contaminated feed or water. Once inside the host, Besnoitia besnoiti invades host cells using specialized organelles called micronemes, rhoptries, and dense granules. Micronemes release proteins involved in host cell recognition and attachment, while rhoptries inject proteins that aid in the invasion process. Dense granules contribute to the establishment of the parasitophorous vacuole, a protective compartment where the parasite resides within the host cell.

    Besnoitia besnoiti primarily infects cattle, causing the disease bovine besnoitiosis. This disease has significant economic implications due to reduced productivity and compromised animal welfare. Infections often occur through direct contact with infected animals or via mechanical transmission by biting insects, such as tabanid flies. It has been reported in various regions, including Europe, Africa, and South America.

    Clinical signs of bovine besnoitiosis vary depending on the stage of the disease. Initial infection may present with mild symptoms, such as fever and respiratory distress. As the infection progresses, characteristic skin lesions develop. These lesions first appear as small, raised, red spots known as maculae, which later progress to form papules and eventually become large, thick, and darkened areas called plaques. The skin lesions can be widespread and predominantly affect the head, neck, shoulders, and limbs. In severe cases, extensive tissue damage and inflammation occur, leading to severe debilitation and even death.

    Besnoitia besnoiti is not limited to cattle; it has been found to infect other ruminants as well. Wild ruminants, such as deer and antelopes, have been identified as potential hosts. However, their role in the epidemiology of bovine besnoitiosis is still being studied.

    Accurate diagnosis of Besnoitia besnoiti infection is essential for disease management and control. Several diagnostic methods are available, including nucleic acid-based techniques and serological testing. Polymerase Chain Reaction (PCR) assays targeting specific genes, such as the Internal Transcribed Spacer 1 (ITS-1) region of ribosomal DNA, have been developed for the detection of Besnoitia besnoiti DNA. These tests can provide sensitive and specific identification of the parasite.

    Serological tests, such as Enzyme-Linked Immunosorbent Assays (ELISAs) or Immunofluorescence Antibody Tests (IFAT), detect specific antibodies produced by the host in response to Besnoitia besnoiti infection. Target antigens used in these tests include BbSRS2 and BbP35, which have been shown to elicit an immune response.

    In conclusion, Besnoitia besnoiti is an apicomplexan protozoan parasite that causes bovine besnoitiosis. It has a complex cellular structure and an intricate life cycle involving both sexual and asexual stages. The disease primarily affects cattle, leading to skin lesions, respiratory distress, and weight loss. Accurate diagnosis of Besnoitia besnoiti infection is crucial for effective disease management, and various diagnostic methods, including nucleic acid-based techniques and serological testing, are available for this purpose.



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