Bovine viral diarrhea virus antibody and antigen (recombinant protein)

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Diagnostic anti-Bovine viral diarrhea virus antibodies pairs and antigen for animal health (animal Bovines/Cattle infectious disease Bovine Viral Diarrhea) testing in ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA and POCT

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Product information

Catalog No.	Description	US $ Price (per mg)
GMP-VT-P053-Ag01	Recombinant Bovine viral diarrhea virus protein	$3090.00
GMP-VT-P053-Ab01	Anti-Bovine viral diarrhea virus mouse monoclonal antibody (mAb)	$3090.00
GMP-VT-P053-Ab02	Anti-Bovine viral diarrhea virus mouse monoclonal antibody (mAb)	$3090.00

Size： 1mg | 10mg | 100mg

Product Description

Cat No.	GMP-VT-P053-Ag01
Product Name	Recombinant Bovine viral diarrhea virus protein
Pathogen	Bovine viral diarrhea virus
Expression platform	E.coli
Isotypes	Recombinant Antigen
Bioactivity validation	Anti-Bovine viral diarrhea virus antibodies binding, Immunogen in Sandwich Elisa, lateral-flow tests, and other immunoassays as control material in Bovine viral diarrhea virus level test of animal Bovines/Cattle infectious disease with Bovine Viral Diarrhea.
Tag	His
Product description	Recombinant Bovine viral diarrhea virus proteinwas expressed in E.coli - based prokaryotic cell expression system and is expressed with 6 HIS tag at the C-terminus.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in Sandwich ELISA, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from sterile PBS, PH 7.4
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Cat No.	GMP-VT-P053-Ab01,GMP-VT-P053-Ab02
Pathogen	Bovine viral diarrhea virus
Product Name	Anti-Bovine viral diarrhea virus mouse monoclonal antibody (mAb)
Expression platform	CHO
Isotypes	Mouse IgG
Bioactivity validation	Recombinant Bovine viral diarrhea virus antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Bovine viral diarrhea virus antibodies in Bovine viral diarrhea virus level test of animal Bovines/Cattle infectious disease with Bovine Viral Diarrhea.
Product description	Anti-Bovine viral diarrhea virus mouse monoclonal antibody (mAb) is a mouse monoclonal antibody produced by CHO technology. The antibody is ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Bovine viral diarrhea virus antibodies./td>
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in Sandwich ELISA, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from sterile PBS, PH 7.4
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Reference

Validation Data

Click to get more Data / Case study about the product.

Pathogen

BVDV is a pestivirus that primarily affects ruminants, including cattle, sheep, goats, and deer. The virus is transmitted through contact with bodily fluids of infected animals, such as nasal secretions, saliva, urine, or feces. Infection can occur through direct contact between animals or indirectly through contaminated feed, water, or equipment.

Clinical manifestations of BVDV infection can vary depending on the strain of the virus, the age and immune status of the infected animal, and other factors. In adult cattle, infection with BVDV commonly causes acute or chronic respiratory disease, fever, diarrhea, and decreased milk production. Additionally, BVDV can lead to immunosuppression, making affected animals more susceptible to secondary bacterial or viral infections.

BVDV infection in pregnant cows can have severe consequences on reproductive outcomes if the fetus is exposed to the virus during early pregnancy. The virus can infect the developing fetus, leading to embryonic or fetal death, abortion, or the birth of persistently infected (PI) calves. PI calves are born with the virus and shed it continuously throughout their lives. They are the main source of viral transmission on farms and pose a significant threat to animal health and welfare.

Structurally, BVDV has a spherical shape and ranges from 40-60 nm in diameter. The virus envelope contains two major structural glycoproteins, E1 and E2, which are involved in virus attachment and entry into host cells. The nucleocapsid protein encapsulates the virus genome, a single positive-stranded RNA molecule that is approximately 12.3 kb in size. The virus genome encodes a polyprotein that is processed by viral and host proteases into individual structural and non-structural proteins.

The non-structural proteins of BVDV, including NS2-3, NS4A, NS4B, NS5A, and NS5B, are involved in viral replication and modulation of host cell functions. NS2-3 is a protease responsible for cleaving the viral polyprotein into individual non-structural proteins. NS4A and NS4B are involved in virus assembly and release. NS5A is a phosphoprotein that plays a role in viral replication and modulation of host cell signaling pathways. NS5B is the RNA-dependent RNA polymerase responsible for viral RNA replication.

Diagnostic methods for BVDV include serological tests such as enzyme-linked immunosorbent assays (ELISA) and agar gel immunodiffusion (AGID), which detect antibodies against the virus in serum or milk samples. Virus isolation is another diagnostic method that involves culturing the virus from clinical samples, such as nasal swabs or blood, in cell culture. Nucleic acid detection methods, such as reverse transcription-polymerase chain reaction (RT-PCR), detect the virus genome in clinical samples. The targeted genes or proteins in these diagnostic tests include the non-structural protein 3 (NS3), the 5' untranslated region (UTR), and the Npro protein.

In conclusion, Bovine viral diarrhea virus is a pestivirus that primarily affects ruminants. The virus can cause significant economic losses in the cattle industry through impacts on reproductive outcomes and reduced productivity. BVDV has a complex structure and expresses several non-structural proteins that are involved in viral replication and modulation of host cell functions. Diagnostic methods for BVDV include serological tests, virus isolation, and nucleic acid detection, with targeted genes or proteins, including NS3, 5' UTR, and Npro.

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