Enzootic Bovine Leukosis Virus/ bovine leukemia virus antibody and antigen (recombinant protein)

Diagnostic anti-Enzootic Bovine Leukosis Virus/ bovine leukemia virus antibodies pairs and antigen for animal health (animal Bovines/Cattle infectious disease enzootic bovine leukosis) testing in ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA and POCT

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Product information

Catalog No. Description US $ Price (per mg)
GMP-VT-P063-Ag01 Recombinant Enzootic Bovine Leukosis Virus/ bovine leukemia virus protein $3090.00
GMP-VT-P063-Ab01 Anti-Enzootic Bovine Leukosis Virus/ bovine leukemia virus mouse monoclonal antibody (mAb) $3090.00
GMP-VT-P063-Ab02 Anti-Enzootic Bovine Leukosis Virus/ bovine leukemia virus mouse monoclonal antibody (mAb) $3090.00

Size: 1mg | 10mg | 100mg



Product Description

Cat No. GMP-VT-P063-Ag01
Product Name Recombinant Enzootic Bovine Leukosis Virus/ bovine leukemia virus protein
Pathogen Enzootic Bovine Leukosis Virus/ bovine leukemia virus
Expression platform E.coli
Isotypes Recombinant Antigen
Bioactivity validation Anti-Enzootic Bovine Leukosis Virus/ bovine leukemia virus antibodies binding, Immunogen in Sandwich Elisa, lateral-flow tests, and other immunoassays as control material in Enzootic Bovine Leukosis Virus/ bovine leukemia virus level test of animal Bovines/Cattle infectious disease with enzootic bovine leukosis.
Tag His
Product description Recombinant Enzootic Bovine Leukosis Virus/ bovine leukemia virus proteinwas expressed in E.coli - based prokaryotic cell expression system and is expressed with 6 HIS tag at the C-terminus.
Purity Purity: ≥95% (SDS-PAGE)
Application Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation Lyophilized from sterile PBS, PH 7.4
Storage Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.


Cat No. GMP-VT-P063-Ab01,GMP-VT-P063-Ab02
Pathogen Enzootic Bovine Leukosis Virus/ bovine leukemia virus
Product Name Anti-Enzootic Bovine Leukosis Virus/ bovine leukemia virus mouse monoclonal antibody (mAb)
Expression platform CHO
Isotypes Mouse IgG
Bioactivity validation Recombinant Enzootic Bovine Leukosis Virus/ bovine leukemia virus antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Enzootic Bovine Leukosis Virus/ bovine leukemia virus antibodies in Enzootic Bovine Leukosis Virus/ bovine leukemia virus level test of animal Bovines/Cattle infectious disease with enzootic bovine leukosis.
Product description Anti-Enzootic Bovine Leukosis Virus/ bovine leukemia virus mouse monoclonal antibody (mAb) is a mouse monoclonal antibody produced by CHO technology. The antibody is ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Enzootic Bovine Leukosis Virus/ bovine leukemia virus antibodies./td>
Purity Purity: ≥95% (SDS-PAGE)
Application Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation Lyophilized from sterile PBS, PH 7.4
Storage Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.


Reference




    Validation Data


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    Pathogen


    Enzootic Bovine Leukosis Virus (EBLV) - An In-Depth Exploration

    Enzootic Bovine Leukosis Virus (EBLV), alternatively referred to as Bovine Leukemia Virus (BLV), is a retrovirus that primarily affects cattle. This virus belongs to the Retroviridae family and the genus Deltaretrovirus, making it an RNA-based virus with the distinctive ability to reverse transcribe its RNA genome into DNA, which is then integrated into the host's genomic material. In this comprehensive overview, we delve into the intricate facets of EBLV, exploring its classification, genetic structure, host specificity, associated diseases, and diagnostic methodologies for precise detection.

    Classification of EBLV/BLV:

    EBLV/BLV is classified as a retrovirus, which distinguishes it from prokaryotic and eukaryotic pathogens. Retroviruses are characterized by their unique replication strategy, involving the conversion of viral RNA into DNA through the action of the enzyme reverse transcriptase. The newly formed viral DNA is then integrated into the host's genomic DNA, thus establishing a permanent genetic presence within the host.

    Pathogen Structure - Genetic Components:

    EBLV/BLV exhibits a well-defined genetic structure consisting of key genes and proteins:

    Envelope Glycoprotein (gp51): The viral envelope glycoprotein, designated as gp51, plays a pivotal role in the virus's interaction with host cells. This protein is responsible for mediating the initial attachment and entry of the virus into target cells.

    Polymerase (pol) Gene: The polymerase gene (pol) encodes a set of enzymes vital for viral replication. Among these, reverse transcriptase is a key player, facilitating the conversion of viral RNA into DNA during the replication cycle.

    Tax Gene: The Tax gene encodes a protein essential for viral replication and the transformation of infected lymphocytes. It plays a central role in the virus's life cycle and its pathogenicity in the host.

    Hosts Infected by EBLV/BLV and Associated Diseases:

    EBLV/BLV predominantly targets cattle, with a primary host range encompassing Bos taurus and Bos indicus species. Once the virus infects these hosts, it leads to the emergence of several related diseases, collectively referred to as bovine leukemia. These diseases encompass:

    Bovine Leukemia: This is the overarching term for the condition, reflecting the viral etiology and the wide range of clinical manifestations in infected cattle.

    Enzootic Bovine Leukosis: This name specifically highlights the endemic nature of the disease in certain cattle populations.

    Bovine Lymphosarcoma: As the virus infects lymphocytes, it can lead to the development of lymphocytic tumors in infected animals, particularly in lymphoid tissues and organs. These tumors can significantly impact the overall health and well-being of the affected cattle.

    The clinical signs of EBLV/BLV infection in cattle may include weight loss, reduced milk production, lymph node enlargement, and a compromised immune system.

    Diagnostic Methods for EBLV/BLV Detection:

    Accurate detection and diagnosis of EBLV/BLV infections are crucial for managing and controlling the disease within cattle herds. Several diagnostic methods have been developed, including those that target viral nucleic acids (RNA or DNA), proteins, and antibodies. Notable diagnostic techniques include:

    Polymerase Chain Reaction (PCR): PCR assays are highly sensitive and specific, allowing for the detection of viral nucleic acids. They often target conserved regions within the viral genome, such as the pol gene. PCR-based tests enable the quantification of viral load and the identification of infected animals.

    Enzyme-Linked Immunosorbent Assay (ELISA): ELISA tests are instrumental in detecting the presence of viral proteins, such as the gp51 envelope glycoprotein, in blood or serum samples. These tests can indicate the current or past infection status of cattle.

    Western Blot: Western blot assays are utilized to confirm the existence of antibodies against EBLV/BLV proteins in serum samples. They are essential for serological testing and distinguishing between infected and non-infected animals.

    Immunohistochemistry: This method is employed to detect viral antigens in tissue samples from affected cattle. It is particularly useful for post-mortem examinations and histopathological analyses.

    Proviral DNA Test: Proviral DNA tests target the integrated viral DNA within the host's genomic material. By identifying this integrated viral DNA, these tests provide a direct and conclusive indicator of infection, even in the absence of detectable viral RNA.

    In summary, EBLV/BLV is a retrovirus with a complex genetic structure that primarily infects cattle, leading to various forms of bovine leukemia. Accurate diagnostic methods are vital for disease control, and these techniques encompass molecular tests, protein assays, and antibody detection methods, which collectively facilitate the precise identification of infected animals and inform disease management strategies within cattle populations.



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