Mannheimia haemolytica Leukotoxin(lktA) antibody and antigen (recombinant protein)

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Diagnostic anti-Mannheimia haemolytica Leukotoxin(lktA) antibodies pairs and antigen for animal health (animal Bovines/Cattle, Ovines/Sheep, Caprine/Goat, Deer infectious disease respiratory syndrome) testing in ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA and POCT

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Product information

Catalog No.	Description	US $ Price (per mg)
GMP-VT-P084-Tg001-Ag01	Recombinant Mannheimia haemolytica Leukotoxin(lktA) protein	$3090.00
GMP-VT-P084-Tg001-Ab01	Anti-Mannheimia haemolytica Leukotoxin(lktA) mouse monoclonal antibody (mAb)	$3090.00
GMP-VT-P084-Tg001-Ab02	Anti-Mannheimia haemolytica Leukotoxin(lktA) mouse monoclonal antibody (mAb)	$3090.00
GMP-VT-P084-Tg001-Ab03	Anti-Mannheimia haemolytica Leukotoxin(lktA) human monoclonal antibody (mAb)	$3090.00
GMP-VT-P084-Tg001-Ab04	Anti-Mannheimia haemolytica Leukotoxin(lktA) human monoclonal antibody (mAb)	$3090.00

Size： 1mg | 10mg | 100mg

Product Description

Cat No.	GMP-VT-P084-Tg001-Ag01
Product Name	Recombinant Mannheimia haemolytica Leukotoxin(lktA) protein
Pathogen	Mannheimia haemolytica
Expression platform	E.coli
Isotypes	Recombinant Antigen
Bioactivity validation	Anti-Mannheimia haemolytica Leukotoxin(lktA) antibodies binding, Immunogen in Sandwich Elisa, lateral-flow tests, and other immunoassays as control material in Mannheimia haemolytica level test of animal Bovines/Cattle, Ovines/Sheep, Caprine/Goat, Deer infectious disease with respiratory syndrome.
Tag	His
Product description	Recombinant Mannheimia haemolytica Leukotoxin(lktA) proteinwas expressed in E.coli - based prokaryotic cell expression system and is expressed with 6 HIS tag at the C-terminus.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from sterile PBS, PH 7.4
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Cat No.	GMP-VT-P084-Tg001-Ab01,GMP-VT-P084-Tg001-Ab02
Pathogen	Mannheimia haemolytica
Product Name	Anti-Mannheimia haemolytica Leukotoxin(lktA) mouse monoclonal antibody (mAb)
Expression platform	CHO
Isotypes	Mouse IgG
Bioactivity validation	Recombinant Mannheimia haemolytica Leukotoxin(lktA) antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Mannheimia haemolytica antibodies in Mannheimia haemolytica level test of animal Bovines/Cattle, Ovines/Sheep, Caprine/Goat, Deer infectious disease with respiratory syndrome.
Product description	Anti-Mannheimia haemolytica Leukotoxin(lktA) mouse monoclonal antibody (mAb) is a mouse monoclonal antibody produced by CHO technology. The antibody is ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Mannheimia haemolytica antibodies.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from sterile PBS, PH 7.4
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Cat No.	GMP-VT-P084-Tg001-Ab03,GMP-VT-P084-Tg001-Ab04
Pathogen	Mannheimia haemolytica
Product Name	Anti-Mannheimia haemolytica Leukotoxin(lktA) human monoclonal antibody (mAb)
Expression platform	CHO
Isotypes	Human lgG1
Bioactivity validation	Recombinant Mannheimia haemolytica Leukotoxin(lktA) antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Mannheimia haemolytica antibodies in Mannheimia haemolytica level test of animal Bovines/Cattle, Ovines/Sheep, Caprine/Goat, Deer infectious disease with respiratory syndrome.
Product description	Anti-Mannheimia haemolytica Leukotoxin(lktA) mouse monoclonal antibody (mAb) is a human monoclonal antibody produced by CHO. The antibody is ELISA validated as capture antibody and detection antibody pair.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from sterile PBS, PH 7.4
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Reference

Validation Data

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Pathogen Information

Mannheimia haemolytica is a Gram-negative bacterium that is responsible for causing respiratory infections in ruminants. Originally classified as Pasteurella haemolytica, this pathogen was later reclassified as Mannheimia haemolytica due to its genetic characteristics, distinct biochemical profile, and pathogenicity.

Mannheimia haemolytica belongs to the bacterial classification, specifically the Pasteurellaceae family. This family is commonly associated with respiratory diseases in animals, particularly in ruminants such as cattle, sheep, and goats. M. haemolytica is an opportunistic pathogen, meaning it is capable of causing disease when the animal's immune system is compromised or when exposed to certain environmental stressors such as transportation or mixing with other animals.

The main virulence factor of Mannheimia haemolytica is the leukotoxin, which is encoded by the lktA gene. This protein is responsible for destroying leukocytes and promoting lung damage in infected animals. The leukotoxin is also capable of eliciting a potent inflammatory response, leading to the accumulation of inflammatory cells in the lungs and increased mucus production, which can further compromise respiratory function.

Mannheimia haemolytica primarily infects ruminants, particularly cattle, sheep, and goats. The pathogen is highly contagious and can be transmitted through direct contact with infected animals, as well as through contaminated feed, water, and equipment. Once the pathogen gains entry into the respiratory tract, it adheres to and colonizes the mucosal surface, eventually leading to tissue damage and subsequent disease.

The primary disease caused by Mannheimia haemolytica is shipping fever, which is a common respiratory disease affecting cattle during transportation. Shipping fever results from a combination of factors, including stress, overcrowding, and exposure to pathogens such as M. haemolytica. Other diseases caused by this pathogen include bovine respiratory disease, enzootic pneumonia, and pneumonic pasteurellosis. These diseases are characterized by a range of clinical signs, including fever, coughing, nasal discharge, and difficulty breathing.

Diagnostic methods for Mannheimia haemolytica include bacterial culture, enzyme-linked immunosorbent assay (ELISA), and polymerase chain reaction (PCR). These techniques target specific genes and proteins, such as the lktA gene and lipopolysaccharides, for accurate identification and diagnosis. Bacterial culture is the gold standard diagnostic method, as it allows for the isolation and identification of the pathogen. However, culture-based methods can be time-consuming and may require specialized laboratory facilities. ELISA and PCR, on the other hand, offer rapid and sensitive diagnostic tools that can be performed in the field or laboratory.

In conclusion, Mannheimia haemolytica is an important pathogen that causes respiratory diseases in ruminants. The pathogen's main virulence factor is the leukotoxin, which is responsible for promoting lung damage and inflammation. Mannheimia haemolytica primarily infects cattle, sheep, and goats and can cause diseases such as shipping fever, bovine respiratory disease, enzootic pneumonia, and pneumonic pasteurellosis. Diagnostic methods for this pathogen include bacterial culture, ELISA, and PCR.

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