Blue ear virus antibody and antigen (recombinant protein)
Diagnostic anti-Blue ear virus antibodies pairs and antigen for animal health (animal Swine/Porcine/Pig infectious disease Porcine reproductive and respiratory syndrome, (PRRS)) testing in ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA and POCT
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Product information
Catalog No. | Description | US $ Price (per mg) |
---|---|---|
GMP-VT-P097-Ag01 | Recombinant Blue ear virus protein | $3090.00 |
GMP-VT-P097-Ab01 | Anti-Blue ear virus mouse monoclonal antibody (mAb) | $3090.00 |
GMP-VT-P097-Ab02 | Anti-Blue ear virus mouse monoclonal antibody (mAb) | $3090.00 |
Size: 1mg | 10mg | 100mg
Product Description
Cat No. | GMP-VT-P097-Ag01 |
Product Name | Recombinant Blue ear virus protein |
Pathogen | Blue ear virus |
Expression platform | E.coli |
Isotypes | Recombinant Antigen |
Bioactivity validation | Anti-Blue ear virus antibodies binding, Immunogen in Sandwich Elisa, lateral-flow tests, and other immunoassays as control material in Blue ear virus level test of animal Swine/Porcine/Pig infectious disease with Porcine reproductive and respiratory syndrome, (PRRS). |
Tag | His | Product description | Recombinant Blue ear virus proteinwas expressed in E.coli - based prokaryotic cell expression system and is expressed with 6 HIS tag at the C-terminus. |
Purity | Purity: ≥95% (SDS-PAGE) |
Application | Paired antibody immunoassay validation in Sandwich ELISA, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays. |
Formulation | Lyophilized from sterile PBS, PH 7.4 |
Storage | Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles. |
Cat No. | GMP-VT-P097-Ab01,GMP-VT-P097-Ab02 |
Pathogen | Blue ear virus |
Product Name | Anti-Blue ear virus mouse monoclonal antibody (mAb) |
Expression platform | CHO |
Isotypes | Mouse IgG |
Bioactivity validation | Recombinant Blue ear virus antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Blue ear virus antibodies in Blue ear virus level test of animal Swine/Porcine/Pig infectious disease with Porcine reproductive and respiratory syndrome, (PRRS). |
Product description | Anti-Blue ear virus mouse monoclonal antibody (mAb) is a mouse monoclonal antibody produced by CHO technology. The antibody is ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Blue ear virus antibodies./td> |
Purity | Purity: ≥95% (SDS-PAGE) |
Application | Paired antibody immunoassay validation in Sandwich ELISA, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays. |
Formulation | Lyophilized from sterile PBS, PH 7.4 |
Storage | Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles. |
Reference
Validation Data
Click to get more Data / Case study about the product.
Pathogen
Blue Ear Virus (BEV), also known as Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) or PRRSV-2, is a type of virus that primarily infects pigs and is a significant cause of economic loss in the swine industry worldwide. The virus was first identified in the United States in 1987 but has since spread to many countries.
BEV belongs to the family Arteriviridae, genus Betaarterivirus, and is a positive-sense, single-stranded RNA virus. The virus has a complex genome consisting of several genes, including ORF1a and ORF1b, which encode various non-structural proteins. Additionally, BEV has four structural proteins encoded by the GP2, E, M, and N genes.
Among the structural genes, the ORF5 gene encoding the major glycoprotein GP5 is the most essential for BEV. The protein is highly antigenic and is considered the primary protective antigen against the virus. The GP5 protein plays an important role in viral replication, assembly, and entry into host cells.
BEV predominantly infects pig populations and causes porcine reproductive and respiratory syndrome (PRRS), a disease characterized by respiratory distress, fever, lethargy, decreased reproductive performance, and immunosuppression in infected animals. The condition can affect piglets, growing pigs, and adult pigs alike.
Molecular diagnostic methods for BEV include polymerase chain reaction (PCR), enzyme-linked immunosorbent assay (ELISA), and indirect fluorescent antibody test (IFAT). These methods typically target specific genes or proteins, such as ORF7, ORF5, and GP5. PCR can detect viral RNA in samples such as nasal swabs, serum, and lung tissues from infected animals. ELISA and IFAT are used to detect antibodies against the virus in the blood serum of infected animals.
Other diagnostic methods for BEV include virus isolation and culturing the virus in vitro. These methods require specialized facilities and expertise but can confirm the presence of the virus and study its characteristics. Additionally, serological tests such as ELISA and immunofluorescence assays (IFA) can detect antibodies against BEV in the blood serum of infected animals.
Several vaccines are available for BEV, primarily based on the GP5 protein or other viral proteins. Vaccination has been shown to reduce the severity of the disease and the spread of the virus in pig populations.
In conclusion, Blue Ear Virus is a significant cause of respiratory and reproductive diseases in pigs worldwide, and it can have a severe impact on the swine industry. The virus primarily infects pigs and is characterized by respiratory distress, decreased reproductive performance, and immunosuppression. Molecular diagnostic methods such as PCR, ELISA, and IFAT are commonly used to diagnose the virus, and several vaccines are available to prevent the disease.
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