African Swine Fever Virus antibody and antigen (recombinant protein)

Diagnostic anti-African Swine Fever Virus antibodies pairs and antigen for animal health (animal Swine/Porcine/Pig infectious disease African swine fever) testing in ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA and POCT

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Product information

Catalog No. Description US $ Price (per mg)
GMP-VT-P107-Ag01 Recombinant African Swine Fever Virus protein $3090.00
GMP-VT-P107-Ab01 Anti-African Swine Fever Virus mouse monoclonal antibody (mAb) $3090.00
GMP-VT-P107-Ab02 Anti-African Swine Fever Virus mouse monoclonal antibody (mAb) $3090.00

Size: 1mg | 10mg | 100mg



Product Description

Cat No. GMP-VT-P107-Ag01
Product Name Recombinant African Swine Fever Virus protein
Pathogen African Swine Fever Virus
Expression platform E.coli
Isotypes Recombinant Antigen
Bioactivity validation Anti-African Swine Fever Virus antibodies binding, Immunogen in Sandwich Elisa, lateral-flow tests, and other immunoassays as control material in African Swine Fever Virus level test of animal Swine/Porcine/Pig infectious disease with African swine fever.
Tag His
Product description Recombinant African Swine Fever Virus proteinwas expressed in E.coli - based prokaryotic cell expression system and is expressed with 6 HIS tag at the C-terminus.
Purity Purity: ≥95% (SDS-PAGE)
Application Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation Lyophilized from sterile PBS, PH 7.4
Storage Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.


Cat No. GMP-VT-P107-Ab01,GMP-VT-P107-Ab02
Pathogen African Swine Fever Virus
Product Name Anti-African Swine Fever Virus mouse monoclonal antibody (mAb)
Expression platform CHO
Isotypes Mouse IgG
Bioactivity validation Recombinant African Swine Fever Virus antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-African Swine Fever Virus antibodies in African Swine Fever Virus level test of animal Swine/Porcine/Pig infectious disease with African swine fever.
Product description Anti-African Swine Fever Virus mouse monoclonal antibody (mAb) is a mouse monoclonal antibody produced by CHO technology. The antibody is ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-African Swine Fever Virus antibodies./td>
Purity Purity: ≥95% (SDS-PAGE)
Application Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation Lyophilized from sterile PBS, PH 7.4
Storage Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.


Reference




    Validation Data


    Click to get more Data / Case study about the product.



    Pathogen


    Introduction:

    African Swine Fever Virus (ASFV) is a substantial concern for the global swine industry. This large, enveloped, double-stranded DNA virus is highly contagious and is notorious for its exclusive pathogenicity to suids, which include both domestic and wild pigs. This article delves into the characteristics, classification, structure, hosts, and diagnostic methods for ASFV.

    Classification and Structure:

    ASFV belongs to the Asfarviridae family within the Asfivirus genus. This classification sets it apart from both eukaryotic and prokaryotic organisms, as it is a virus. The virus exhibits a complex, multi-layered structure, encapsulating a large, linear, double-stranded DNA genome.

    One of the distinctive features of ASFV is its extensive genomic repertoire, encoding over 150 proteins. Among the key genes and proteins, the p72 gene (B646L) stands out as the major capsid protein, playing a pivotal role in maintaining the virus's structural integrity. Other critical proteins include p32 (B602L), which is involved in viral DNA replication; p54 (E183L), essential for viral DNA synthesis; and p30 (H240R), contributing to the virus assembly process.

    Hosts and Associated Disease:

    ASFV is a specialist pathogen with a predilection for suids, targeting both domestic pigs (Sus scrofa domesticus) and wild boars (Sus scrofa). The disease caused by ASFV is referred to as African Swine Fever. It manifests with a spectrum of clinical signs and outcomes, making it a multifaceted threat to the swine industry.

    Clinical signs of African Swine Fever include elevated body temperatures, anorexia, and hemorrhagic symptoms within the skin and internal organs. Perhaps most alarming is the virus's capacity to induce high mortality rates, often reaching 100% in affected populations. These dire outcomes, combined with associated economic repercussions due to mass culling and trade restrictions, underscore the gravity of ASFV as a pathogen.

    Diagnostic Methods:

    Timely detection and accurate diagnosis of ASFV are of paramount importance for the swine industry and veterinary authorities. Various diagnostic methods are employed for this purpose, often targeting specific genes and proteins associated with ASFV. These methods include:

    Polymerase Chain Reaction (PCR): PCR assays target various genomic regions, with the p72 gene (B646L) and the p72-p32 gene region being common targets. PCR allows for the detection and amplification of ASFV DNA, facilitating rapid and sensitive diagnosis.

    Real-time PCR (qPCR): Real-time PCR offers a quantitative approach, enabling the precise measurement of viral DNA levels in samples. This method is particularly useful in monitoring disease progression and evaluating the efficacy of control measures.

    Loop-Mediated Isothermal Amplification (LAMP): LAMP assays target specific genomic segments of ASFV. This isothermal amplification technique is characterized by its simplicity and rapid results, making it valuable for on-site testing in resource-limited settings.

    Enzyme-Linked Immunosorbent Assay (ELISA): ELISA is primarily employed for detecting antibodies against ASFV in swine populations. While it is a valuable tool for serological surveillance, it may not be as effective in the early stages of infection.

    Virus Isolation: This method involves the culture of ASFV in specialized cell lines. Virus isolation is not only used for diagnostic purposes but is also crucial for research, strain characterization, and vaccine development.

    Conclusion:

    In summary, African Swine Fever Virus (ASFV) is a formidable pathogen with a devastating impact on the swine industry. Its unique host specificity, complex structure, and extensive genomic repertoire make it a challenging adversary. Early detection and accurate diagnosis, facilitated by nucleic acid-based diagnostic methods, are vital for effective control and containment of this highly contagious virus. In the ongoing efforts to combat African Swine Fever, understanding the virus at a molecular level and implementing rigorous biosecurity measures are paramount for safeguarding global pork production and trade.



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