Actinobacillus pleuropneumoniae serotype 2 antibody and antigen (recombinant protein)

Diagnostic anti-Actinobacillus pleuropneumoniae serotype 2 antibodies pairs and antigen for animal health (animal Swine/Porcine/Pig infectious disease swine pleuropneumonia) testing in ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA and POCT

Target products collectionGo to Swine/Porcine/Pig disease testing products collection >>


Product information

Catalog No. Description US $ Price (per mg)
GMP-VT-P123-Ag01 Recombinant Actinobacillus pleuropneumoniae serotype 2 protein $3090.00
GMP-VT-P123-Ab01 Anti-Actinobacillus pleuropneumoniae serotype 2 mouse monoclonal antibody (mAb) $3090.00
GMP-VT-P123-Ab02 Anti-Actinobacillus pleuropneumoniae serotype 2 mouse monoclonal antibody (mAb) $3090.00

Size: 1mg | 10mg | 100mg



Product Description

Cat No. GMP-VT-P123-Ag01
Product Name Recombinant Actinobacillus pleuropneumoniae serotype 2 protein
Pathogen Actinobacillus pleuropneumoniae serotype 2
Expression platform E.coli
Isotypes Recombinant Antigen
Bioactivity validation Anti-Actinobacillus pleuropneumoniae serotype 2 antibodies binding, Immunogen in Sandwich Elisa, lateral-flow tests, and other immunoassays as control material in Actinobacillus pleuropneumoniae serotype 2 level test of animal Swine/Porcine/Pig infectious disease with swine pleuropneumonia.
Tag His
Product description Recombinant Actinobacillus pleuropneumoniae serotype 2 proteinwas expressed in E.coli - based prokaryotic cell expression system and is expressed with 6 HIS tag at the C-terminus.
Purity Purity: ≥95% (SDS-PAGE)
Application Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation Lyophilized from sterile PBS, PH 7.4
Storage Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.


Cat No. GMP-VT-P123-Ab01,GMP-VT-P123-Ab02
Pathogen Actinobacillus pleuropneumoniae serotype 2
Product Name Anti-Actinobacillus pleuropneumoniae serotype 2 mouse monoclonal antibody (mAb)
Expression platform CHO
Isotypes Mouse IgG
Bioactivity validation Recombinant Actinobacillus pleuropneumoniae serotype 2 antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Actinobacillus pleuropneumoniae serotype 2 antibodies in Actinobacillus pleuropneumoniae serotype 2 level test of animal Swine/Porcine/Pig infectious disease with swine pleuropneumonia.
Product description Anti-Actinobacillus pleuropneumoniae serotype 2 mouse monoclonal antibody (mAb) is a mouse monoclonal antibody produced by CHO technology. The antibody is ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Actinobacillus pleuropneumoniae serotype 2 antibodies./td>
Purity Purity: ≥95% (SDS-PAGE)
Application Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation Lyophilized from sterile PBS, PH 7.4
Storage Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.


Reference




    Validation Data


    Click to get more Data / Case study about the product.



    Pathogen


    Of the numerous pathogens that infect pigs, Actinobacillus pleuropneumoniae serotype 2 stands out as one of the most important bacterial pathogens responsible for causing porcine pleuropneumonia. Being a bacterium belonging to the family Pasteurellaceae, Actinobacillus pleuropneumoniae is characterized by its small, non-motile, and non-spore-forming rod-shaped cells. The pathogen measures between 0.5 and 2.0 micrometers in size.

    The pathogenicity of Actinobacillus pleuropneumoniae involves several genes and proteins that contribute to the virulence and pathogenesis of the disease. One crucial gene is the Apx toxin gene, which encodes various virulence factors that help the bacteria evade the host immune system and cause disease. Other genes such as capsular polysaccharide genes and lipopolysaccharide genes contribute to bacterial adhesion and colonization on host tissues.

    Actinobacillus pleuropneumoniae primarily affects pigs and is a highly contagious respiratory disease. The infection can be widespread through the herd and has severe economic repercussions for pig farmers worldwide. Clinical symptoms of the disease include fever, cough, nasal discharge, and difficulty breathing. In severe cases, pleurisy, lung abscess, and septicemia have also been reported, which may result in high morbidity and mortality rates.

    Diagnostic methods for Actinobacillus pleuropneumoniae include bacterial culture, polymerase chain reaction (PCR), immunofluorescence assays, and enzyme-linked immunosorbent assays (ELISAs). Bacterial culture of Actinobacillus pleuropneumoniae can be difficult and requires special media and conditions. However, PCR testing has emerged as an alternative diagnostic method that offers high sensitivity and specificity. The most commonly targeted gene in PCR assays is the Apx toxin gene.

    Immunofluorescence assays and ELISAs are serological diagnostic tools that detect antibodies against Actinobacillus pleuropneumoniae, indicating a previous infection. These assays measure antibody levels in serum samples and can differentiate between vaccinated animals and infected ones.

    The control of Actinobacillus pleuropneumoniae infections involves the implementation of preventative strategies such as vaccination, biosecurity measures, and appropriate use of antibiotics. Vaccines targeting the Apx toxins have shown to provide protection against the pathogen, reducing the morbidity and mortality rates. However, the development of effective vaccines for all serotypes of Actinobacillus pleuropneumoniae remains a challenge.

    In conclusion, Actinobacillus pleuropneumoniae serotype 2 is a significant bacterial pathogen affecting pig populations worldwide, leading to severe economic losses. Its pathogenicity is supported by crucial genes and proteins, including the Apx toxin gene responsible for encoding virulence factors. Diagnostic methods, including PCR, immunofluorescence assays, and ELISAs, play a crucial role in early detection, treatment, and prevention of the disease.



    About GDU


    GDU

    GDU helps global diagnostic partners in high quality of raw material discovery, development, and application. GDU believes in Protein&antibody Innovation for more reliable diagnostic solutions.