Avian leukosis virus antibody and antigen (recombinant protein)

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Diagnostic anti-Avian leukosis virus antibodies pairs and antigen for animal health (animal Chicken infectious disease Lymphoid leukosis) testing in ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA and POCT

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Product information

Catalog No.	Description	US $ Price (per mg)
GMP-VT-P172-Ag01	Recombinant Avian leukosis virus protein	$3090.00
GMP-VT-P172-Ab01	Anti-Avian leukosis virus mouse monoclonal antibody (mAb)	$3090.00
GMP-VT-P172-Ab02	Anti-Avian leukosis virus mouse monoclonal antibody (mAb)	$3090.00

Size： 1mg | 10mg | 100mg

Product Description

Cat No.	GMP-VT-P172-Ag01
Product Name	Recombinant Avian leukosis virus protein
Pathogen	Avian leukosis virus
Expression platform	E.coli
Isotypes	Recombinant Antigen
Bioactivity validation	Anti-Avian leukosis virus antibodies binding, Immunogen in Sandwich Elisa, lateral-flow tests, and other immunoassays as control material in Avian leukosis virus level test of animal Chicken infectious disease with Lymphoid leukosis.
Tag	His
Product description	Recombinant Avian leukosis virus proteinwas expressed in E.coli - based prokaryotic cell expression system and is expressed with 6 HIS tag at the C-terminus.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in Sandwich ELISA, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from GM's Protein Stability Buffer2 (PSB2,Confidential Ingredients) or PBS (pH7.4); For PSB2, reconstituted with 0.9% sodium chloride; For PBS, reconstituted with ddH2O.
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Cat No.	GMP-VT-P172-Ab01,GMP-VT-P172-Ab02
Pathogen	Avian leukosis virus
Product Name	Anti-Avian leukosis virus mouse monoclonal antibody (mAb)
Expression platform	CHO
Isotypes	Mouse IgG
Bioactivity validation	Recombinant Avian leukosis virus antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Avian leukosis virus antibodies in Avian leukosis virus level test of animal Chicken infectious disease with Lymphoid leukosis.
Product description	Anti-Avian leukosis virus mouse monoclonal antibody (mAb) is a mouse monoclonal antibody produced by CHO technology. The antibody is ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Avian leukosis virus antibodies./td>
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in Sandwich ELISA, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from GM's Protein Stability Buffer2 (PSB2,Confidential Ingredients) or PBS (pH7.4); For PSB2, reconstituted with 0.9% sodium chloride; For PBS, reconstituted with ddH2O.
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Reference

Validation Data

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Pathogen

Avian leukosis virus (ALV) is a retrovirus that infects primarily poultry, including chickens and turkeys, inducing various forms of cancer and immunosuppression. The virus' replication and pathogenesis rely on the integration of its viral genome into the host's chromosomal DNA, making it difficult to eradicate once established.

ALV is classified as a type of virus, more specifically, a retrovirus. Retroviruses are unique in that they integrate their genetic material into the host's DNA and subsequently use the host's machinery to reproduce. ALV's genetic structure is composed of several genes and proteins, including gag, pro, pol, and env. The gag gene encodes the structural proteins that form the mature virus particle; the pro gene encodes enzymes required for post-translational modification of the structural proteins; the pol gene encodes other essential enzymes required for reverse transcribing the viral RNA and integrating it into the host's genome; and the env gene encodes the surface glycoproteins responsible for receptor binding.

The virus primarily infects juvenile birds and spreads from hen to chick via the egg. However, horizontal transmission can occur via contact with body fluids or contaminated objects. The infected bird may remain dormant but eventually develops symptoms that include anemia, weight loss, dyspnea (shortness of breath), poor feathering, and a decrease in egg production.

ALV can cause multiple diseases in its avian hosts, including lymphoid leukemia, myeloid leukemia, erythroblastosis, and other tumors. Lymphoid leukemia manifests as a cancer of the white blood cells, specifically the lymphocytes. It is the most common form of leukemia caused by ALV. Myeloid leukemia affects the white blood cells and the bone marrow. Erythroblastosis is a condition in which the red blood cell precursors overproduce, leading to anemia. ALV can also cause immunosuppression, increasing the susceptibility of affected birds to other infections.

The diagnosis of ALV infection requires specialized methods, such as Enzyme-Linked Immunosorbent Assay (ELISA), Polymerase Chain Reaction (PCR), and virus isolation. The ELISA test detects viral antigens in sera or tissues, while the PCR-based method detects viral DNA or RNA sequences in the same samples. Virus isolation is the most definitive method of detecting ALV and involves growing the virus in culture and then confirming its identity. Furthermore, nucleic acid-based diagnostic tools like reverse transcriptase PCR (RT-PCR) and loop-mediated isothermal amplification (LAMP) can detect ALV with high sensitivity and specificity.

Prevention and control of ALV involve

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