Escherichia coli O157:H7 antibody and antigen (recombinant protein)

Diagnostic anti-Escherichia coli O157:H7 antibodies pairs and antigen for animal health (animal Cat/Feline, Dog/Canine, Rabbit, Bovines/Cattle, Equine/Horse, Ovines/Sheep, Caprine/Goat, Fish, Swine/Porcine/Pig, Avian/Bird/Poultry infectious disease diarrhea, hemorrhagic colitis, and hemolytic-uremic syndrome) testing in ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA and POCT

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Product information

Catalog No. Description US $ Price (per mg)
GMP-VT-P290-Ag01 Recombinant Escherichia coli O157:H7 protein $3090.00
GMP-VT-P290-Ab01 Anti-Escherichia coli O157:H7 mouse monoclonal antibody (mAb) $3090.00
GMP-VT-P290-Ab02 Anti-Escherichia coli O157:H7 mouse monoclonal antibody (mAb) $3090.00

Size: 1mg | 10mg | 100mg



Product Description

Cat No. GMP-VT-P290-Ag01
Product Name Recombinant Escherichia coli O157:H7 protein
Pathogen Escherichia coli O157:H7
Expression platform E.coli
Isotypes Recombinant Antigen
Bioactivity validation Anti-Escherichia coli O157:H7 antibodies binding, Immunogen in Sandwich Elisa, lateral-flow tests, and other immunoassays as control material in Escherichia coli O157:H7 level test of animal Cat/Feline, Dog/Canine, Rabbit, Bovines/Cattle, Equine/Horse, Ovines/Sheep, Caprine/Goat, Fish, Swine/Porcine/Pig, Avian/Bird/Poultry infectious disease with diarrhea, hemorrhagic colitis, and hemolytic-uremic syndrome.
Tag His
Product description Recombinant Escherichia coli O157:H7 proteinwas expressed in E.coli - based prokaryotic cell expression system and is expressed with 6 HIS tag at the C-terminus.
Purity Purity: ≥95% (SDS-PAGE)
Application Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation Lyophilized from sterile PBS, PH 7.4
Storage Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.


Cat No. GMP-VT-P290-Ab01,GMP-VT-P290-Ab02
Pathogen Escherichia coli O157:H7
Product Name Anti-Escherichia coli O157:H7 mouse monoclonal antibody (mAb)
Expression platform CHO
Isotypes Mouse IgG
Bioactivity validation Recombinant Escherichia coli O157:H7 antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Escherichia coli O157:H7 antibodies in Escherichia coli O157:H7 level test of animal Cat/Feline, Dog/Canine, Rabbit, Bovines/Cattle, Equine/Horse, Ovines/Sheep, Caprine/Goat, Fish, Swine/Porcine/Pig, Avian/Bird/Poultry infectious disease with diarrhea, hemorrhagic colitis, and hemolytic-uremic syndrome.
Product description Anti-Escherichia coli O157:H7 mouse monoclonal antibody (mAb) is a mouse monoclonal antibody produced by CHO technology. The antibody is ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Escherichia coli O157:H7 antibodies./td>
Purity Purity: ≥95% (SDS-PAGE)
Application Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation Lyophilized from sterile PBS, PH 7.4
Storage Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.


Reference




    Validation Data


    Click to get more Data / Case study about the product.



    Pathogen


    Escherichia coli O157:H7, also known as E. coli O157:H7 or simply O157, is a bacterial pathogen that is responsible for causing a range of debilitating and potentially fatal diseases. This pathogen has become the focus of intense scrutiny in recent years due to its emergence as a significant cause of foodborne illness.

    Pathogen Classification:

    E. coli O157:H7 is a prokaryotic bacterium belonging to the family Enterobacteriaceae and the species Escherichia coli. E. coli bacteria are gram-negative, rod-shaped bacteria that are commonly found in the intestines of humans and animals.

    Structure and Virulence Factors:

    The structure of E. coli O157:H7 consists of a single cell that measures approximately 2-4 micrometers in length and 0.5-1 micrometer in width. The bacteria have a thin layer of peptidoglycan and an outer membrane composed of lipopolysaccharides. Unlike other strains of E. coli, this pathogen produces several virulence factors that allow it to cause disease in humans. The primary virulence factor is Shiga toxin, known as Stx, which is encoded by the stx gene. This protein is responsible for the cytotoxic effects of the bacteria, including damage to the intestinal lining, kidney failure, and other complications.

    Hosts and Diseases:

    E. coli O157:H7 primarily infects cattle, which can carry the bacteria asymptomatically. When humans consume contaminated food products, such as undercooked beef or raw milk, they can become infected with this pathogen, which can lead to a range of illnesses. These illnesses can include diarrhea, abdominal cramping, and fever, which typically resolve within a week. However, in some cases, severe complications such as hemorrhagic colitis, hemolytic uremic syndrome (HUS), and thrombotic thrombocytopenic purpura (TTP) can develop, leading to kidney failure, anemia, and even death.

    Diagnostic Methods:

    The diagnosis of E. coli O157:H7 infection relies on the use of laboratory techniques to detect the bacteria or its virulence factors in clinical specimens such as stool samples. The traditional culture method involves inoculating a sample onto agar media that contains specific nutrients needed for E. coli growth. PCR-based assays are also commonly used to detect the presence of stx genes in clinical specimens. Serotyping based on the O and H antigens of the bacteria is also employed to confirm the identification of E. coli O157:H7. Other nucleic acid amplification methods such as LAMP and real-time PCR have demonstrated potential advantages over traditional methods, including faster turnaround times and higher sensitivity. ELISA for the Stx protein is another diagnostic method available.

    Prevention and Control:

    Prevention and control of E. coli O157:H7 infections primarily rely on food safety measures, such as proper handling, storage, and cooking of meat products, washing fruits, vegetables and hands before handling food. Additionally, surveillance programs to monitor and track outbreak occurrences are crucial in preventing further transmission. Once diagnosed, patients with severe infections may require hospitalization and supportive care, including fluid and electrolyte replacement, nutrition support, and potentially dialysis.

    In conclusion, Escherichia coli O157:H7 is a bacterial pathogen that poses significant health risks to humans, particularly in cases of contaminated food ingestion. Early detection, prevention measures, and appropriate treatment of contamination are crucial in managing outbreaks and reducing the incidence of this pathogen.



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