Human MMP25/MMP-25/MMP20 ORF/cDNA clone-Lentivirus plasmid (NM_022468.5)

Cat. No.: pGMLV002178
Size: 10 µg
Concentration: generally 0.5ug/ul, usually not less than 0.3ug/ul
Leading Time: 3-7 working days

Pre-made Human MMP25/MMP-25/MMP20 Lentiviral expression plasmid for MMP25 lentivirus packaging, MMP25 lentivirus production, overexpression stable cell line development, cell transient transfection and gene delivery targeting T/B/NK cells, macrophages, cardiomyocytes, hepatocytes, and neurons.

Our GM-Lentiviral vector is seamlessly integrated with the GM lentivirus packaging system. Discover more about the GM lentivirus packaging system.

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Product Description

Catalog ID pGMLV002178
Gene Name MMP25
Accession Number NM_022468.5
Gene ID 64386
Species Human
Product Type Lentivirus plasmid (overexpression)
Insert Length 1689 bp
Gene Alias MMP-25,MMP20,MMP20A,MMPL1,MT-MMP 6,MT-MMP6,MT6-MMP,MT6MMP,MTMMP6
Fluorescent Reporter ZsGreen
Mammalian Cell Selection Puromyocin
Fusion Tag 3xflag (C-Terminal)
Promoter CMV
Resistance Amplicin
ORF Nucleotide Sequence ATGCGGCTGCGGCTCCGGCTTCTGGCGCTGCTGCTTCTGCTGCTGGCACCGCCCGCGCGCGCCCCGAAGCCCTCGGCGCAGGACGTGAGCCTGGGCGTGGACTGGCTGACTCGCTATGGTTACCTGCCGCCACCCCACCCTGCCCAGGCCCAGCTGCAGAGCCCTGAGAAGTTGCGCGATGCCATCAAAGTCATGCAGAGGTTCGCGGGGCTGCCGGAGACCGGCCGCATGGACCCAGGGACAGTGGCCACCATGCGTAAGCCCCGCTGCTCCCTGCCTGACGTGCTGGGGGTGGCGGGGCTGGTCAGGCGGCGTCGCCGGTACGCTCTGAGCGGCAGCGTGTGGAAGAAGCGAACCCTGACATGGAGGGTACGTTCCTTCCCCCAGAGCTCCCAGCTGAGCCAGGAGACCGTGCGGGTCCTCATGAGCTATGCCCTGATGGCCTGGGGCATGGAGTCAGGCCTCACATTTCATGAGGTGGATTCCCCCCAGGGCCAGGAGCCCGACATCCTCATCGACTTTGCCCGCGCCTTCCACCAGGACAGCTACCCCTTCGACGGGTTGGGGGGCACCCTAGCCCATGCCTTCTTCCCTGGGGAGCACCCCATCTCCGGGGACACTCACTTTGACGATGAGGAGACCTGGACTTTTGGGTCAAAAGACGGCGAGGGGACCGACCTGTTTGCCGTGGCTGTCCATGAGTTTGGCCACGCCCTGGGCCTGGGCCACTCCTCAGCCCCCAACTCCATTATGAGGCCCTTCTACCAGGGTCCGGTGGGCGACCCTGACAAGTACCGCCTGTCTCAGGATGACCGCGATGGCCTGCAGCAACTCTATGGGAAGGCGCCCCAAACCCCATATGACAAGCCCACAAGGAAACCCCTGGCTCCTCCGCCCCAGCCCCCGGCCTCGCCCACACACAGCCCATCCTTCCCCATCCCTGATCGATGTGAGGGCAATTTTGACGCCATCGCCAACATCCGAGGGGAAACTTTCTTCTTCAAAGGCCCCTGGTTCTGGCGCCTCCAGCCCTCCGGACAGCTGGTGTCCCCGCGACCCGCACGGCTGCACCGCTTCTGGGAGGGGCTGCCCGCCCAGGTGAGGGTGGTGCAGGCCGCCTATGCTCGGCACCGAGACGGCCGAATCCTCCTCTTTAGCGGGCCCCAGTTCTGGGTGTTCCAGGACCGGCAGCTGGAGGGCGGGGCGCGGCCGCTCACGGAGCTGGGGCTGCCCCCGGGAGAGGAGGTGGACGCCGTGTTCTCGTGGCCACAGAACGGGAAGACCTACCTGGTCCGCGGCCGGCAGTACTGGCGCTACGACGAGGCGGCGGCGCGCCCGGACCCCGGCTACCCTCGCGACCTGAGCCTCTGGGAAGGCGCGCCCCCCTCCCCTGACGATGTCACCGTCAGCAACGCAGGTGACACCTACTTCTTCAAGGGCGCCCACTACTGGCGCTTCCCCAAGAACAGCATCAAGACCGAGCCGGACGCCCCCCAGCCCATGGGGCCCAACTGGCTGGACTGCCCCGCCCCGAGCTCTGGTCCCCGCGCCCCCAGGCCCCCCAAAGCGACCCCCGTGTCCGAAACCTGCGATTGTCAGTGCGAGCTCAACCAGGCCGCAGGACGTTGGCCTGCTCCCATCCCGCTGCTCCTCTTGCCCCTGCTGGTGGGGGGTGTAGCCTCCCGCTGA
ORF Protein Sequence MRLRLRLLALLLLLLAPPARAPKPSAQDVSLGVDWLTRYGYLPPPHPAQAQLQSPEKLRDAIKVMQRFAGLPETGRMDPGTVATMRKPRCSLPDVLGVAGLVRRRRRYALSGSVWKKRTLTWRVRSFPQSSQLSQETVRVLMSYALMAWGMESGLTFHEVDSPQGQEPDILIDFARAFHQDSYPFDGLGGTLAHAFFPGEHPISGDTHFDDEETWTFGSKDGEGTDLFAVAVHEFGHALGLGHSSAPNSIMRPFYQGPVGDPDKYRLSQDDRDGLQQLYGKAPQTPYDKPTRKPLAPPPQPPASPTHSPSFPIPDRCEGNFDAIANIRGETFFFKGPWFWRLQPSGQLVSPRPARLHRFWEGLPAQVRVVQAAYARHRDGRILLFSGPQFWVFQDRQLEGGARPLTELGLPPGEEVDAVFSWPQNGKTYLVRGRQYWRYDEAAARPDPGYPRDLSLWEGAPPSPDDVTVSNAGDTYFFKGAHYWRFPKNSIKTEPDAPQPMGPNWLDCPAPSSGPRAPRPPKATPVSETCDCQCELNQAAGRWPAPIPLLLLPLLVGGVASR

Reference




    Data / case study


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    Associated products


    Category Cat No. Products Name
    Target Antibody GM-Tg-g-MP0820-Ab Anti-MMP25/ MMP-25/ MMP20 monoclonal antibody
    Target Antigen GM-Tg-g-MP0820-Ag MMP25 VLP (virus-like particle)
    ORF Viral Vector pGMLV002178 Human MMP25 Lentivirus plasmid
    ORF Viral Vector vGMLV002178 Human MMP25 Lentivirus particle


    Target information

    Target ID GM-MP0820
    Target Name MMP25
    Gene ID 64386, 240047, 700486, 302963, 101097941, 490046, 531092, 100068942
    Gene Symbol and Synonyms F730048C11Rik,Leukolysin,MMP-25,MMP20,MMP20A,MMP25,MMPL1,MT-MMP 6,MT-MMP6,MT6-MMP,MT6MMP,MTMMP6
    Uniprot Accession Q9NPA2
    Uniprot Entry Name MMP25_HUMAN
    Protein Sub-location Transmembrane Protein
    Category Not Available
    Disease Not Available
    Gene Ensembl ENSG00000008516
    Target Classification Not Available

    Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily, attached to the plasma membrane via a glycosylphosphatidyl inositol anchor. In response to bacterial infection or inflammation, the encoded protein is thought to inactivate alpha-1 proteinase inhibitor, a major tissue protectant against proteolytic enzymes released by activated neutrophils, facilitating the transendothelial migration of neutrophils to inflammatory sites. The encoded protein may also play a role in tumor invasion and metastasis through activation of MMP2. The gene has previously been referred to as MMP20 but has been renamed MMP25. [provided by RefSeq, Jul 2008]



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