Ornithobacterium rhinotracheale antibody and antigen (recombinant protein)

Diagnostic anti-Ornithobacterium rhinotracheale antibodies pairs and antigen for animal health (animal Avian/Bird/Poultry infectious disease ornithobacteriosis) testing in ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA and POCT

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Product information

Catalog No. Description US $ Price (per mg)
GMP-VT-P181-Ag01 Recombinant Ornithobacterium rhinotracheale protein $3090.00
GMP-VT-P181-Ab01 Anti-Ornithobacterium rhinotracheale mouse monoclonal antibody (mAb) $3090.00
GMP-VT-P181-Ab02 Anti-Ornithobacterium rhinotracheale mouse monoclonal antibody (mAb) $3090.00

Size: 1mg | 10mg | 100mg



Product Description

Cat No. GMP-VT-P181-Ag01
Product Name Recombinant Ornithobacterium rhinotracheale protein
Pathogen Ornithobacterium rhinotracheale
Expression platform E.coli
Isotypes Recombinant Antigen
Bioactivity validation Anti-Ornithobacterium rhinotracheale antibodies binding, Immunogen in Sandwich Elisa, lateral-flow tests, and other immunoassays as control material in Ornithobacterium rhinotracheale level test of animal Avian/Bird/Poultry infectious disease with ornithobacteriosis.
Tag His
Product description Recombinant Ornithobacterium rhinotracheale proteinwas expressed in E.coli - based prokaryotic cell expression system and is expressed with 6 HIS tag at the C-terminus.
Purity Purity: ≥95% (SDS-PAGE)
Application Paired antibody immunoassay validation in Sandwich ELISA, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation Lyophilized from sterile PBS, PH 7.4
Storage Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.


Cat No. GMP-VT-P181-Ab01,GMP-VT-P181-Ab02
Pathogen Ornithobacterium rhinotracheale
Product Name Anti-Ornithobacterium rhinotracheale mouse monoclonal antibody (mAb)
Expression platform CHO
Isotypes Mouse IgG
Bioactivity validation Recombinant Ornithobacterium rhinotracheale antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Ornithobacterium rhinotracheale antibodies in Ornithobacterium rhinotracheale level test of animal Avian/Bird/Poultry infectious disease with ornithobacteriosis.
Product description Anti-Ornithobacterium rhinotracheale mouse monoclonal antibody (mAb) is a mouse monoclonal antibody produced by CHO technology. The antibody is ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Ornithobacterium rhinotracheale antibodies./td>
Purity Purity: ≥95% (SDS-PAGE)
Application Paired antibody immunoassay validation in Sandwich ELISA, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation Lyophilized from sterile PBS, PH 7.4
Storage Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.


Reference




    Validation Data


    Click to get more Data / Case study about the product.



    Pathogen


    Ornithobacterium rhinotracheale (ORT) is a Gram-negative bacterium that belongs to the family Pasteurellaceae. It is a respiratory pathogen of poultry species, causing serious conditions such as avian infectious bronchitis (IB), avian bordetellosis, and avian coryza. Infections with ORT can lead to several clinical signs which include nasal discharge, respiratory distress, coughing, sneezing, and decreased egg production in infected birds.

    ORT was first isolated in the 1980s from turkeys with respiratory disease, and since then, it has been reported in various parts of the world, including Europe, Asia, South Africa, and America. This wide distribution of ORT may be attributed to its ability to survive in different environments and hosts. It's worth noting that this pathogen is specific to birds and does not pose any significant threat to humans.

    ORT is a small, non-motile bacterium that measures approximately 1-2 μm in length and 0.5 μm in diameter. Its cell wall contains peptidoglycan, which provides structural support, and lipopolysaccharides (LPS), which are essential for virulence. ORT also has an outer membrane that is made up of various proteins, with the most prominent being the outer membrane protein A (OmpA). The OmpA protein is involved in the pathogenicity of ORT by promoting bacterial adhesion to host cells.

    One of the diagnostic methods used to identify ORT infections is through culture. ORT grows best on blood agar plates at a temperature range of 37-42°C, where colonies appear after 24-48 hours of incubation. However, culturing ORT may be difficult due to its fastidious growth requirements, and as a result, other diagnostic methods have been developed to identify the pathogen.

    Polymerase Chain Reaction (PCR) is a molecular diagnostic tool that has been used to successfully detect ORT infections. PCR amplifies specific genes found within the bacterial genome. For example, the 16S rRNA gene has been used as a target in identifying ORT strains. Besides the 16S rRNA gene, other genes such as the sodA gene and hsf have also been used as targets for diagnosing ORT infections.

    Enzyme-linked immunosorbent assay (ELISA) is another commonly used diagnostic tool for the detection of ORT infections. ELISA can detect specific antigens or antibodies present within infected tissues, fluids, or sera. In ORT, the OmpA protein and LPS are the primary targets used in ELISA-based diagnosis. A positive diagnosis in ELISA indicates the presence of these proteins within the sample being analyzed.

    Immunohistochemistry (IHC) is another diagnostic method used for detecting ORT infections. IHC is a technique that uses antibodies to detect antigens within infected tissues. Like ELISA, IHC primarily targets antigens associated with ORT, such as OmpA and LPS.

    In conclusion, ORT is a respiratory pathogen of poultry species that causes respiratory diseases such as avian infectious bronchitis (IB), avian bordetellosis, and avian coryza. The pathogen's cell wall is made up of peptidoglycan and LPS, and its outer membrane contains various proteins, including OmpA. Diagnostic methods for ORT infections include culture, PCR, ELISA, and immunohistochemistry. PCR targets genes such as the 16S rRNA, sodA, and hsf genes, while ELISA and immunohistochemistry target proteins such as OmpA and LPS.



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