Escherichia coli CS31A antibody and antigen (recombinant protein)

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Diagnostic anti-Escherichia coli CS31A antibodies pairs and antigen for animal health (animal Bovines/Cattle, Ovines/Sheep, Caprine/Goat, Deer infectious disease septicemic colibacillosis) testing in ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA and POCT

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Product information

Catalog No.	Description	US $ Price (per mg)
GMP-VT-P289-Ag01	Recombinant Escherichia coli CS31A protein	$3090.00
GMP-VT-P289-Ab01	Anti-Escherichia coli CS31A mouse monoclonal antibody (mAb)	$3090.00
GMP-VT-P289-Ab02	Anti-Escherichia coli CS31A mouse monoclonal antibody (mAb)	$3090.00

Size： 1mg | 10mg | 100mg

Product Description

Cat No.	GMP-VT-P289-Ag01
Product Name	Recombinant Escherichia coli CS31A protein
Pathogen	Escherichia coli CS31A
Expression platform	E.coli
Isotypes	Recombinant Antigen
Bioactivity validation	Anti-Escherichia coli CS31A antibodies binding, Immunogen in Sandwich Elisa, lateral-flow tests, and other immunoassays as control material in Escherichia coli CS31A level test of animal Bovines/Cattle, Ovines/Sheep, Caprine/Goat, Deer infectious disease with septicemic colibacillosis.
Tag	His
Product description	Recombinant Escherichia coli CS31A proteinwas expressed in E.coli - based prokaryotic cell expression system and is expressed with 6 HIS tag at the C-terminus.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in Sandwich ELISA, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from GM's Protein Stability Buffer2 (PSB2,Confidential Ingredients) or PBS (pH7.4); For PSB2, reconstituted with 0.9% sodium chloride; For PBS, reconstituted with ddH2O.
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Cat No.	GMP-VT-P289-Ab01,GMP-VT-P289-Ab02
Pathogen	Escherichia coli CS31A
Product Name	Anti-Escherichia coli CS31A mouse monoclonal antibody (mAb)
Expression platform	CHO
Isotypes	Mouse IgG
Bioactivity validation	Recombinant Escherichia coli CS31A antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Escherichia coli CS31A antibodies in Escherichia coli CS31A level test of animal Bovines/Cattle, Ovines/Sheep, Caprine/Goat, Deer infectious disease with septicemic colibacillosis.
Product description	Anti-Escherichia coli CS31A mouse monoclonal antibody (mAb) is a mouse monoclonal antibody produced by CHO technology. The antibody is ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Escherichia coli CS31A antibodies./td>
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in Sandwich ELISA, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from GM's Protein Stability Buffer2 (PSB2,Confidential Ingredients) or PBS (pH7.4); For PSB2, reconstituted with 0.9% sodium chloride; For PBS, reconstituted with ddH2O.
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Reference

Validation Data

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Pathogen

Escherichia coli CS31A, a pathogenic strain of Escherichia coli, is known for causing urinary tract infections (UTIs) and meningitis in humans. It is a gram-negative bacterium that colonizes in the intestine of humans and animals, and it is associated with various diseases globally.

As a prokaryotic organism, Escherichia coli CS31A consists of a single, circular chromosome and a small amount of plasmids. The genome of this pathogen encodes various virulence factors such as fimbriae, which are hair-like appendages that allow the bacterium to attach to host cells and tissues. Colibactin is another virulence factor produced by Escherichia coli CS31A, which is produced by the pks gene. This genotoxic molecule has been demonstrated to cause DNA damage and is therefore associated with the development of colon cancer.

The adhesive properties of fimbriae also play a crucial role in the pathogenesis of Escherichia coli CS31A infections. A thin layer of the fimbriae protein coats the bacterial surface and mediates bacterial-cell interaction. Escherichia coli CS31A also possesses other adhesins like lipopolysaccharides that aid in binding to host tissues, evading the immune system, and accessing nutrients in the intestinal environment.

Escherichia coli CS31A primarily affects human hosts, leading to various diseases. UTIs caused by Escherichia coli CS31A result from the bacterium’s adherence to the uroepithelium and subsequent production of virulence factors, including fimbriae and colibactin which contribute to the pathogenesis of infection. Additionally, meningitis can occur if the bacteria enter the bloodstream from sites of infection and disperse to the central nervous system. Escherichia coli CS31A also infects animals such as pigs, cattle, and poultry, causing septicemia, enteritis, and other infections.

The detection of Escherichia coli CS31A is essential for effective management of the resulting diseases. Diagnostic methods include bacterial culture, molecular assays, and serological tests. Bacterial culture enables bacterial growth and isolation, which is identified based on colony morphology, gram stain, and biochemical tests. Molecular assays, including PCR, offer rapid detection and confirmation of bacterial presence by targeting specific DNA sequences associated with the pathogen. Additionally, serological testing for antibody detection using the enzyme-linked immunosorbent assay technique is commonly used to assess past or present infection.

Overall, Escherichia coli CS31A is a pathogenic strain of E. coli that poses a significant threat to public health. Its virulence factors, including fimbriae and colibactin, enable it to infect various hosts, causing UTIs, meningitis, and other diseases. Effective diagnosis and management of Escherichia coli CS31A infections are vital for reducing the incidence and severity of diseases associated with this pathogen.

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