Human AKT1/AKT/ PKB ORF/cDNA clone-Lentivirus plasmid (NM_005163)

Product information

Catalog No.	Product Name	Plasmid Grade	Plasmid quantity
pGMLV000222	Human AKT1 Lentivirus plasmid	Research Grade	10mg, 50mg, 100mg, 500mg, >1g
		GMP-like Grade	10mg, 50mg, 100mg, 500mg, >1g
		High Quality (HQ) Grade
		Seed	5ug

Product Description

Catalog ID	pGMLV000222
Gene Name	AKT1
Accession Number	NM_005163
Gene ID	207
Species	Human
Product Type	Lentivirus plasmid (overexpression)
Insert Length	1443 bp
Gene Alias	AKT, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA
Fluorescent Reporter	Luciferase
Mammalian Cell Selection	Puromyocinmyocin
Fusion Tag	3xflag (C-Terminal)
Promoter	CMV
Resistance	Amplicin

Reference

Data / case study

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Associated products

Category	Cat No.	Products Name
Target Antibody	GM-Tg-g-T67619-Ab	Anti-AKT1 monoclonal antibody
Target Antigen	GM-Tg-g-T67619-Ag	AKT1 protein
ORF Viral Vector	pGMLV000222	Human AKT1 Lentivirus plasmid
ORF Viral Vector	pGMLV000429	Human AKT1 Lentivirus plasmid
ORF Viral Vector	pGMLV000909	Rat Akt1 Lentivirus plasmid
ORF Viral Vector	pGMLV001602	Human AKT1 Lentivirus plasmid
ORF Viral Vector	pGMPC000431	Human AKT1 Mammalian (Non-Viral Vector) plasmid
ORF Viral Vector	pGMPC000689	Rat Akt1 Mammalian (Non-Viral Vector) plasmid
ORF Viral Vector	pGMAP000133	Human AKT1 Adenovirus plasmid
ORF Viral Vector	vGMLV000222	Human AKT1 Lentivirus particle
ORF Viral Vector	vGMLV000429	Human AKT1 Lentivirus particle
ORF Viral Vector	vGMLV000909	Rat Akt1 Lentivirus particle
ORF Viral Vector	vGMLV001602	Human AKT1 Lentivirus particle
ORF Viral Vector	vGMAP000133	Human AKT1 Adenovirus particle

Target information

Target ID	GM-T67619
Target Name	AKT1
Gene ID	207, 11651, 697747, 24185, 101087931, 490878, 280991, 100061130
Gene Symbol and Synonyms	AKT,AKT1,LTR-akt,PKB,PKB-ALPHA,PKB/Akt,PKBalpha,PRKBA,RAC,RAC-ALPHA
Uniprot Accession	P31749
Uniprot Entry Name	AKT1_HUMAN
Protein Sub-location	Introcelluar Protein
Category	Therapeutics Target
Disease	Non-Small Cell Lung Cancer
Gene Ensembl	ENSG00000142208
Target Classification	Kinase

This gene encodes one of the three members of the human AKT serine-threonine protein kinase family which are often referred to as protein kinase B alpha, beta, and gamma. These highly similar AKT proteins all have an N-terminal pleckstrin homology domain, a serine/threonine-specific kinase domain and a C-terminal regulatory domain. These proteins are phosphorylated by phosphoinositide 3-kinase (PI3K). AKT/PI3K forms a key component of many signalling pathways that involve the binding of membrane-bound ligands such as receptor tyrosine kinases, G-protein coupled receptors, and integrin-linked kinase. These AKT proteins therefore regulate a wide variety of cellular functions including cell proliferation, survival, metabolism, and angiogenesis in both normal and malignant cells. AKT proteins are recruited to the cell membrane by phosphatidylinositol 3,4,5-trisphosphate (PIP3) after phosphorylation of phosphatidylinositol 4,5-bisphosphate (PIP2) by PI3K. Subsequent phosphorylation of both threonine residue 308 and serine residue 473 is required for full activation of the AKT1 protein encoded by this gene. Phosphorylation of additional residues also occurs, for example, in response to insulin growth factor-1 and epidermal growth factor. Protein phosphatases act as negative regulators of AKT proteins by dephosphorylating AKT or PIP3. The PI3K/AKT signalling pathway is crucial for tumor cell survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating AKT1 which then phosphorylates and inactivates components of the apoptotic machinery. AKT proteins also participate in the mammalian target of rapamycin (mTOR) signalling pathway which controls the assembly of the eukaryotic translation initiation factor 4F (eIF4E) complex and this pathway, in addition to responding to extracellular signals from growth factors and cytokines, is disregulated in many cancers. Mutations in this gene are associated with multiple types of cancer and excessive tissue growth including Proteus syndrome and Cowden syndrome 6, and breast, colorectal, and ovarian cancers. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2020]

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