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COVID-19, a novel pathogenic coronavirus (2019-nCoV or SARS-CoV2) induced disease, emerged in China and spread globally rapidly.

GeneMedi produces COVID-19 related anti-2019 nCoV(SARS2 coronavirus) antibodies as shown below:
Anti-2019-nCoV N protein (Nucleocapsid protein) antibody: GMP-V-2019nCoV-NAb001
Anti-2019-nCoV NP scFv-Fc antibody: GMP-V-2019nCoV-NAb002
Anti-2019-nCoV NP monoclonal mouse antibody: GMP-V-2019nCoV-NAb003 , GMP-V-2019nCoV-NAb004
Anti-2019-nCoV-Spike protein(S protein, S1 protein, Spike RBD) antibody: GMP-V-2019nCoV-S1Ab001, GMP-V-2019nCoV-S1Ab002

COVID-19 related antibodies (Human IgG1) were expressed in mammalian cell(human cells, HEK293).

All the antibodies of 2019 nCoV are suitable for in functional ELISA, and other immunoassays in dignostics. The antibody can act as a capture antibody and detection antibody.

GeneMedi has submitted a preprints review about COVID-19 and 2019-nCoV.

Click here for the full-text reading:

Viral vector-based vaccine; DNA-based vaccine; RNA based vaccine - A landscape for vaccine technology against infectious disease, COVID-19 and tumor.

An Insight of comparison between COVID-19 (2019-nCoV disease) and SARS in pathology and pathogenesis

Landscape Coronavirus Disease 2019 test (COVID-19 test) in vitro -- A comparison of PCR vs Immunoassay vs Crispr-Based test

In the mission of helping global scientists for the development of diagnosis and drug against 2019 nCoV(SARS2 coronavirus), GeneMedi scientists will keep moving.


2019 nCoV (SARS2 coronavirus) Antibodies for COVID-19


Cat No. Products Name Host specics Source (Expression Host) Bioactivity validation Order
GMP-V-2019nCoV-NAb001 Anti-2019-nCoV NP human monoclonal antibody human
IgG
Mammalian
(human cell)
N protein binding, ELISA validated as capture antibody and detection antibody.
Pair recommendation with GMP-V-2019nCoV-NAb002 , GMP-V-2019nCoV-NAb003 , GMP-V-2019nCoV-NAb004.
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GMP-V-2019nCoV-NAb002 Anti-2019-nCoV NP human
scFv-Fc antibody
Scfv Mammalian
(human cell)
N protein binding, ELISA validated as capture antibody and detection antibody.
Pair recommendation with GMP-V-2019nCoV-NAb001, GMP-V-2019nCoV-NAb003 , GMP-V-2019nCoV-NAb004.
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GMP-V-2019nCoV-NAb003 Anti-2019-nCoV NP mouse monoclonal antibody(mAb) Mouse
IgG
Hybridoma N protein binding, ELISA validated as capture antibody and detection antibody.
Pair recommendation with GMP-V-2019nCoV-NAb001, GMP-V-2019nCoV-NAb002, GMP-V-2019nCoV-NAb004.
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GMP-V-2019nCoV-NAb004 Anti-2019-nCoV NP mouse monoclonal antibody(mAb) Mouse
IgG
Hybridoma N protein binding, ELISA validated as capture antibody and detection antibody.
Pair recommendation with GMP-V-2019nCoV-NAb001, GMP-V-2019nCoV-NAb002 , GMP-V-2019nCoV-NAb003.
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GMP-V-2019nCoV-S1Ab001 Anti-2019-nCoV Spike (S1 protein) human monoclonal antibody human
IgG
Mammalian
(human cell)
S-RBD protein binding, ELISA validated Picture loading failed.
GMP-V-2019nCoV-S1Ab002 Anti-2019-nCoV Spike (S1 protein) human monoclonal antibody human
IgG
Mammalian
(human cell)
S-RBD protein binding, ELISA validated, Western Blot validated Picture loading failed.

GeneMedi's SARS-CoV-2 NP Antibody Pair And Stability Validation In Sandwich ELISA

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Figure. GeneMedi's SARS-CoV2 NP antibody(Nab) pair validation with NP antigen(GMP-V-2019nCoVN002>) in sandwich ELISA. Nabs were either in PBS solution (stocked in -20 for 7days) or lyophilized (stock at room temperature for 7 days). GeneMedi's lyophilized antibodies resented excellent stability in the room temperature condition.

Abbreviation Description
Nab001 GMP-V-2019nCoV-NAb001
Nab002 GMP-V-2019nCoV-NAb002
Nab003 GMP-V-2019nCoV-NAb003
Nab004 GMP-V-2019nCoV-NAb004
PBS liquid in PBS buffer
lyophilized lyophilized and stock at room temperature for 7 days before reconstitution
NP SARS-CoV-2 NP atntigen (GMP-V-2019nCoV-N002)
Response Normalized Normalized by Max OD450


Recombinant 2019 nCoV (SARS2 coronavirus) Antigens reagents


Cat No. Antigen Name of 2019-nCoV(SARS-CoV-2) Source
(Expression Host)
Tag Bioactivity validation Order
GMP-V-2019nCoV-N002 Recombinant 2019-nCoV(SARS-CoV-2) Nucleocapsid Protein (N protein,N-His tag)Picture loading failed. E.coli N-His Picture loading failed.
GMP-V-2019nCoV-SRBD001 Recombinant 2019-nCoV(SARS-CoV-2) Spike Protein (RBD, C-His Tag)Picture loading failed. Mamamlian (human cell) C-His ACE2 binding; Picture loading failed.
GMP-V-2019nCoV-SRBD002 Recombinant 2019-nCoV(SARS-CoV-2) Spike Protein (RBD, C-mFC Tag)Picture loading failed. Mamamlian (human cell) C-mFc ACE2 binding; Picture loading failed.
GMP-V-2019nCoV-S1001 Recombinant 2019-nCoV(SARS-CoV-2) Spike Protein (S1, His Tag) Baculovirus-Insect Cells C-His Picture loading failed.
GMP-V-2019nCoV-S1S2001 Recombinant 2019-nCoV(SARS-CoV-2) Spike Protein (S1+S2 ECD, His tag) Baculovirus-Insect Cells C-His Picture loading failed.
GMP-V-2019nCoV-E001 Recombinant 2019-nCoV(SARS-CoV-2) Envelope Protein (E protein,His Tag) E.coli N-His Enzyme activity validation in biochemical assay Picture loading failed.
GMP-H-ACE2001 Recombinant human soluble ACE2 protein (soluble hACE2,extracellular hACE2,C-His) Mamamlian (human cell) C-His Spike(S-RBD) protein binding Picture loading failed.
GMP-H-ACE2002 Recombinant human soluble ACE2 protein (soluble hACE2, extracellular hACE2, C-FC) Mamamlian (human cell) C-Fc Spike(S-RBD) protein binding Picture loading failed.
GMP-V-2019nCoV-Mpro001 Recombinant 2019-nCoV(SARS-CoV-2) Main Proteinase (Mpro,3CLpro, His Tag) E.coli N-His Enzyme activity validation in biochemical assay Picture loading failed.
GMP-V-2019nCoV-PLpro001 Recombinant 2019-nCoV(SARS-CoV-2) papain-like proteinase (PLpro, Nsp3, His Tag) E.coli N-His In Testing Picture loading failed.
GMP-V-2019nCoV-RdRP001 Recombinant 2019-nCoV(SARS-CoV-2) RNA-directed RNA polymerase(RdRP, Nsp12, His Tag) E.coli C-His In Testing Picture loading failed.
GMP-V-2019nCoV-Nsp3X-01 Recombinant 2019-nCoV(SARS-CoV-2)Nsp3-X domain(Macro domain, His tag) E.coli N-His In Testing Picture loading failed.
GMP-V-2019nCoV-Nsp10-01 Recombinant 2019-nCoV(SARS-CoV-2) nsp10 (nsp10-CysHis,GFL protein,His Tag) E.coli N-His In Testing Picture loading failed.
GMP-V-2019nCoV-Nsp16-01 Recombinant 2019-nCoV(SARS-CoV-2) nsp16 (nsp16-OMT,2'-o-MT,His Tag) E.coli N-His In Testing Picture loading failed.

GeneMedi also provides pre-made the lentivirus, adenovirus and AAV vector for the gene ORF plasmids of 2019 nCoV (SARS2 coronavirus):
https://www.genemedi.net/i/2019-ncov-gene-vectors


About SARS-CoV-2 (2019nCoV, novel Coronavirus) Spike protein, S1 Protein, Spike S1-NTD, Spike S1-CTD, Spike-RBD and Spike trimer protein.


1. SARS-CoV-2 (2019nCoV) Spike protein: SARS-CoV-2, a newly emerged pathogen spreading worldwide. The transmembrane spike (S) glycoprotein of SARS-CoV-2 that forms homotrimers protruding from the viral surface is known to mediate coronavirus entry into host cells. It has been reported that spike protein can bind with high affinity to human ACE2 and uses it as an entry receptor to invade target cells.

2. SARS-CoV-2 (2019nCoV) spike S1 protein: The spike protein is a large type I transmembrane glycoprotein comprises two functional subunits, S1 and S2. S1 subunit of spike protein is responsible for binding to the host cell receptor. S2 subunit is responsible for fusion of the viral and cellular membranes.

3. SARS-CoV-2 (2019nCoV) spike S1-NTD and S1-CTD: For most coronaviruses, the N-terminal domain (NTD) of the S1 subunit attaches to cellular carbohydrates and the C-terminal domain of S1 (S1-CTD) binds to a cellular protein receptor. Carbohydrate binding by the S1 N-terminal domain is thought to keep the virus in close proximity to the host cell surface, whereas engagement of specific protein receptors by the S1-CTD is thought to initiate a series of conformational changes in the spike that ultimately result in membrane fusion and delivery of the viral genome to the cytosol.

4. SARS-CoV-2 (2019nCoV) Spike RBD: S1 subunit of spike protein contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor.

5. SARS-CoV-2 (2019nCoV) Spike trimer protein: The transmembrane spike (S) glycoprotein of SARS-CoV-2 usually forms homotrimers protruding from the viral surface. S trimers are extensively decorated with N-linked glycans that are important for proper folding and for modulating accessibility to host proteases and neutralizing Abs. It has been found that S glycoprotein trimers in highly pathogenic human coronaviruses appear to exist in partially opened states, while they remain largely closed in human coronaviruses associated with common colds.

About SARS-CoV-2 (2019nCoV, novel Coronavirus) Envelop protein (Coronavirus E protein)


1. SARS-CoV-2 (2019nCoV) E protein: E protein is the smallest major structural proteins. It has a N-terminal ectodomain and a C-terminal endodomain with ion channel activity. During the replication cycle, E protein is abundantly expressed inside the infected cell, but only a small portion is incorporated into the virus envelope. The majority of the protein participates in viral assembly and budding. E protein is important in virus production and maturation. Recombinant CoVs without E have been shown to exhibit significantly reduced viral titres, crippled viral maturation, or yield incompetent progeny.

About SARS-CoV-2 (2019nCoV, novel Coronavirus) membrane protein (Coronavirus M protein)


1. SARS-CoV-2 (2019nCoV) M protein: Coronavirus M protein is believed to define the shape of the viral envelope,which contains three transmembrane domains. It has a small N-terminal glycosylated ectodomain and a much larger C-terminal endodomain that extends 6–8 nm into the viral particle. M protein usualy exists as a dimer, and may adopt two different conformations allowing it to promote membrane curvature as well as bind to the nucleocapsid.

About SARS-CoV-2 (2019nCoV, novel Coronavirus) Nucleocapsid protein (Coronavirus N protein)


1. SARS-CoV-2 (2019nCoV) N protein: Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. N protein packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.

About SARS-CoV-2 (2019nCoV, novel Coronavirus)Non-structure protein (Nsp1-Nsp16)


2019nCoV contains 16 Non-structure protein (Nsp1-Nsp16) that may be drugable targets for antiviral compounds discovery against COVID-191.

Non-structure protein(NSP)information of SARS-CoV-2 (2019nCoV)1
Non-structure proteins Starting position (aa) Ending position (aa) Length (aa)
nsp1 1 180 180
(leader protein)
nsp2 181 818 638
nsp3 819 2763 1945
(Papain-Like proteinase, PLpro)
nsp4 2764 3263 500
nsp5 3264 3569 306
(Mpro, Main proteinase, 3C-like proteinase)
nsp635703859290
nsp73860394283
nsp839434140198
nsp941414253113
nsp10 4254 4392 139
(growth-factor-like protein)
nsp1243935324932
(RdRP,NA-dependent RNA polymerase)
nsp1353255925601
(RNA 5'-triphosphatase)
nsp1459266452527
(3'-to-5' exonuclease)
nsp1564536798346
(endoRNAse)
nsp1667997096298
(2’O-MTase, 2'-O-ribose methyltransferase)

1. Nsp3: Nsp3 (200 kDa) is the largest protein encoded by the coronavirus (CoV) genome. Nsp3 is an essential component of the replication and transcription complex. It comprises various domains, the organization of which differs between CoV genera, due to duplication or absence of some domains. However, the N-terminal region of the Nsp3 is highly conserved among CoV, containing a ubiquitin-like (Ubl) globular fold followed by a flexible, extended acidic-domain (AC domain) rich in glutamic acid (38%). Next to the AC domain is a catalytically active ADP-ribose-1"-phosphatase (ADRP, app-1"-pase) domain (also called macro domain or X domain) thought to play a role during synthesis of viral subgenomic RNAs. SARS Unique Domain (SUD), a domain not yet identified in other coronaviruses from alphacoronavirus and betacoronavirus, follows next. The SUD domain binds oligonucleotides known to form G-quadruplexes. Downstream of the SUD domain is a second Ubl domain and the catalytically active PLpro domain that proteolytically processes the Nsp1/2, Nsp2/3 and Nsp3/4 cleavage sites. Downstream of PLpro are found a nucleic acid-binding domain (NAB) with a nucleic acid chaperon function, which is conserved in betacoronavirus and gammacoronavirus, and one uncharacterized domain termed the marker domain (G2M). Following the G2M are two predicted double-pass transmembrane domains (TM1–2 and TM3–4), a putative metal binding region (ZN) and the Y domain of unknown function (subdomains Y1–3).

2. Nsp5: Nsp5 protease (3CLpro; Mpro) mediates processing at 11 distinct cleavage sites, including its own autoproteolysis, and is essential for virus replication. Nsp5 exhibits a conserved three-domain structure and catalytic residues.

3. Nsp10: Nsp10 (18 kDa) is well conserved among coronaviruses and encoded by ORF1a. It's thought to serve as an important multifunctional cofactor in replication. Nsp10 was shown to interact with itself, as well as with Nsp1, Nsp7, Nsp14, and Nsp16. The important role of Nsp10 is responsible for RNA synthesis. It was shown that a murine hepatitis virus (MHV) temperature-sensitive mutant carrying a non-synonymous mutation in the Nsp10 coding sequence had a defect in minus-strand RNA synthesis at non-permissive temperatures.

4. Nsp12: Nsp12 (102 kDa) is a multidomain RNA polymerase, which is the most conserved protein in coronaviruses. Nsp12 contains an RNA-dependent RNA polymerase (RdRp) domain in its C-terminal, which is essential for the viral replication and transcription.

5. Nsp16: Nsp16 is an SAM-dependent nucleoside-2’O-methyl-transferase (2’O-MTase). The mRNA cap for coronaviruses is completed by Nsp16, which ensures formation of a protective cap-1 structure that prevent recognition by either MDA5 or IFIT proteins. Finally, the NSP16/NSP10 complex finishes coronavirus capping process permitting viral infection with reduced host recognition.

About COVID-19 pandemic, Coronavirus (Coronavirus) and genome of SARS-CoV-2 (2019nCoV)

COVID-19 pandemic is caused by 2019nCoV (SARS-CoV-2, a novel coronavirus) infection.The 2019-nCoV genome was annotated to possess 14 ORFs encoding 27 proteins1.

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Figure. Schematic representative of genome structure and encoded gene of SARS-CoV-2 (2019nCoV)1

Table.Genome Annotation of 2019-nCoV1
Gene name of 2019nCoV (SARS-CoV-2, a novel coronavirus) Coding region(nt) Protein length(aa)
orf1a266-134834405
orf1ab266-13468, 13468-215557096
S21563-253841273
3a25393-26220275
3b25814-2588222
26183-2628132
E (envelope protei)26245-2647275
M (matrix protein)26523-27191222
p627202-2738761
7a27394-27759121
7b27756-2788743
8b27894-28259121
9b28284-2857797
N(nucleocapsid)28274-29533419
orf1428734-2895573

Collection of COVID-19 landscape knowledge base


Viral vector-based vaccine; DNA-based vaccine; RNA based vaccine - A landscape for vaccine technology against infectious disease, COVID-19 and tumor.

COVID-19 landscape Knowledge Base

An Insight of comparison between COVID-19 (2019-nCoV disease) and SARS in pathology and pathogenesis

Landscape Coronavirus Disease 2019 test (COVID-19 test) in vitro -- A comparison of PCR vs Immunoassay vs Crispr-Based test



References

1. Wu, A. et al. Genome Composition and Divergence of the Novel Coronavirus (2019-nCoV) Originating in China. Cell Host Microbe, doi:10.1016/j.chom.2020.02.001 (2020).




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