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Package Catalog No. Price(In USD) Qty (Quantity) Sum(In USD)
1mg GMP-V-2019nCoV-PLpro001-1mg 5990
10mg GMP-V-2019nCoV-PLpro001-10mg 38790
100mg GMP-V-2019nCoV-PLpro001-100mg 258000
≥100mg GMP-V-2019nCoV-PLpro001-xmg Inquiry
Shipping Cost: 760.00
Total:



Description

Accession Number QHD43415.1
Host specics 2019-nCoV(SARS-CoV-2, SARS2-coronavirus)
Source (Expression Host)E.coli
TagN-His
Products description Recombinant 2019-nCoV(SARS-CoV-2, SARS2-coronavirus) papain-like Proteinase (PLpro, PL Proteinase,Nsp3)
was expressed in E.coli-based prokaryotic cell expression system and the target gene encoding Glu1564-IIe1877
was expressed with 6 HIS tag at the N-terminus.
Bioactivity validationIn Testing
PurityPurity: ≥95% (SDS-PAGE)
applicationImmunogen in Elisa,lateral-flow tests,and other immunoassays;Standard substance
The antigen can also be used in drug discovery including antibody screening and lead compound candidates assay.
predicted Molecular Mass38.6kD
FormulationLyophilized from sterile PBS, PH 7.4
StorageStore at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

2019nCoV (SARS-CoV-2) Papain-Like Proteinase (PLpro)


About Coronavirus Papain-Like Proteinase (PLpro)

COVID-19 pandemic is caused by 2019nCoV (SARS-CoV-2) infection. 2019nCoV contains 16 Non-structure protein (Nsp1-Nsp16) that may be drugable targets for antiviral compounds discovery against COVID-191.

2019nCoV (SARS-CoV-2) Papain-Like Proteinase(PLpro) is included in Nsp3. The coronavirus Papain-Like Proteinase(PLpro) has been found as a nucleic acid-binding domain (NAB) with a nucleic acid chaperon function, which is conserved in coronavirus,and one uncharacterized domain termed the marker domain (G2M). Following the G2M are two predicted double-pass transmembrane domains (TM1–2 and TM3–4), a putative metal binding region (ZN) and the Y domain of unknown function (subdomains Y1–3)1,2.

About Nsp3

Nsp3: Nsp3 (200 kDa) is the largest protein encoded by the coronavirus genome (SARS-CoV-2, SARS-CoV, MERS, etc.). Nsp3 is an essential component of the replication and transcription complex. It comprises various domains, the organization of which differs between coronavirus genera, due to duplication or absence of some domains. However, the N-terminal region of the Nsp3 is highly conserved among coronavirus, containing a ubiquitin-like (Ubl) globular fold followed by a flexible, extended acidic-domain (AC domain) rich in glutamic acid (38%). Next to the AC domain is a catalytically active ADP-ribose-1"-phosphatase (ADRP, app-1"-pase) domain (also called macro domain or X domain) thought to play a role during synthesis of viral subgenomic RNAs. SARS Unique Domain (SUD), a domain not yet identified in other coronaviruses from alphacoronavirus and betacoronavirus, follows next. The SUD domain binds oligonucleotides known to form G-quadruplexes. Downstream of the SUD domain is a second Ubl domain and the catalytically active PLpro domain that proteolytically processes the Nsp1/2, Nsp2/3 and Nsp3/4 cleavage sites1,2

High-throughput screening (HTS) assay development of Coronavirus PLpro for antiviral compounds

HTS Approach: Fluorescence resonance energy transfer (FRET) assay for time-dependent kinetic analysis
FRET Substrate: (E-EDANS)RELNGGAPI(K-DABCYL)S (Synthetic)

Simple 2019nCoV (SARS-CoV-2) Papain-Like Proteinase(PLpro) activity assay develoement Protocol2: 2019nCoV (SARS-CoV-2) Papain-Like Proteinase(PLpro) activity assay develoement Protocol article pdf download

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References

1. Gordon, D. E. et al. A SARS-CoV-2-Human Protein-Protein Interaction Map Reveals Drug Targets and Potential Drug-Repurposing. BioRxiv, doi:10.1101/2020.03.22.002386 (2020).
2. Wojdyla, J. A. et al. Papain-like protease 1 from transmissible gastroenteritis virus: crystal structure and enzymatic activity toward viral and cellular substrates. J Virol 84, 10063-10073, doi:10.1128/JVI.00898-10 (2010).


Recombinant 2019 nCoV (SARS2 coronavirus) Antigens reagents


Cat No. Antigen Name of 2019-nCoV(SARS-CoV-2) Source
(Expression Host)
Tag Bioactivity validation Order
GMP-V-2019nCoV-N002 Recombinant 2019-nCoV(SARS-CoV-2)
Nucleocapsid Protein (N protein,N-His tag) Picture loading failed.
E.coli N-His Picture loading failed.
GMP-V-2019nCoV-SRBD001 Recombinant 2019-nCoV(SARS-CoV-2)
Spike Protein (RBD, C-His Tag)Picture loading failed.
Mamamlian (human cell) C-His ACE2 binding; Picture loading failed.
GMP-V-2019nCoV-SRBD002 Recombinant 2019-nCoV(SARS-CoV-2)
Spike Protein (RBD, C-mFC Tag)Picture loading failed.
Mamamlian (human cell) C-mFc ACE2 binding; Picture loading failed.
GMP-V-2019nCoV-S1001 Recombinant 2019-nCoV(SARS-CoV-2)
Spike Protein (S1, His Tag)
Baculovirus-Insect Cells C-His Picture loading failed.
GMP-V-2019nCoV-S1S2001 Recombinant 2019-nCoV(SARS-CoV-2)
Spike Protein (S1+S2 ECD, His tag)
Baculovirus-Insect Cells C-His Picture loading failed.
GMP-V-2019nCoV-E001 Recombinant 2019-nCoV(SARS-CoV-2)
Envelope Protein (E protein,His Tag)
E.coli N-His Picture loading failed.
GMP-H-ACE2001 Recombinant human soluble ACE2 protein
(soluble hACE2,extracellular hACE2,C-His)
Mamamlian (human cell) C-His Spike(S-RBD) protein binding Picture loading failed.
GMP-H-ACE2002 Recombinant human soluble ACE2 protein
(soluble hACE2, extracellular hACE2, C-FC)
Mamamlian (human cell) C-Fc Spike(S-RBD) protein binding Picture loading failed.
GMP-V-2019nCoV-Mpro001 Recombinant 2019-nCoV(SARS-CoV-2)
Main Proteinase (Mpro, 3CLpro, His Tag)
E.coli N-His Enzyme activity validation in biochemical assay Picture loading failed.
GMP-V-2019nCoV-PLpro001 Recombinant 2019-nCoV(SARS-CoV-2)
papain-like proteinase (PLpro, Nsp3, His Tag)
E.coli N-His In Testing Picture loading failed.
GMP-V-2019nCoV-RdRP001 Recombinant 2019-nCoV(SARS-CoV-2)
RNA-directed RNA polymerase(RdRP, Nsp12, His Tag)
E.coli C-His In Testing Picture loading failed.
GMP-V-2019nCoV-Nsp3X-01 Recombinant 2019-nCoV(SARS-CoV-2)
Nsp3-X domain(Macro domain, His tag)
E.coli N-His In Testing Picture loading failed.
GMP-V-2019nCoV-Nsp10-01 Recombinant 2019-nCoV(SARS-CoV-2)
nsp10 (nsp10-CysHis,GFL protein,His Tag)
E.coli N-His In Testing Picture loading failed.
GMP-V-2019nCoV-Nsp16-01 Recombinant 2019-nCoV(SARS-CoV-2)
nsp16 (nsp16-OMT,2'-o-MT,His Tag)
E.coli N-His In Testing Picture loading failed.

2019 nCoV (SARS2 coronavirus) Antibodies for COVID-19


Cat No. Products Name Host specics Source (Expression Host) Bioactivity validation Order
GMP-V-2019nCoV-NAb001 Anti-2019-nCoV NP human monoclonal antibody human
IgG
Mammalian
(human cell)
N protein binding, ELISA validated as capture antibody and detection antibody.
Pair recommendation with GMP-V-2019nCoV-NAb002 , GMP-V-2019nCoV-NAb003 , GMP-V-2019nCoV-NAb004.
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GMP-V-2019nCoV-NAb002 Anti-2019-nCoV NP human
scFv-Fc antibody
Scfv Mammalian
(human cell)
N protein binding, ELISA validated as capture antibody and detection antibody.
Pair recommendation with GMP-V-2019nCoV-NAb001, GMP-V-2019nCoV-NAb003 , GMP-V-2019nCoV-NAb004.
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GMP-V-2019nCoV-NAb003 Anti-2019-nCoV NP mouse monoclonal antibody(mAb) Mouse
IgG
Hybridoma N protein binding, ELISA validated as capture antibody and detection antibody.
Pair recommendation with GMP-V-2019nCoV-NAb001, GMP-V-2019nCoV-NAb002, GMP-V-2019nCoV-NAb004.
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GMP-V-2019nCoV-NAb004 Anti-2019-nCoV NP mouse monoclonal antibody(mAb) Mouse
IgG
Hybridoma N protein binding, ELISA validated as capture antibody and detection antibody.
Pair recommendation with GMP-V-2019nCoV-NAb001, GMP-V-2019nCoV-NAb002 , GMP-V-2019nCoV-NAb003.
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GMP-V-2019nCoV-S1Ab001 Anti-2019-nCoV Spike (S1 protein) human monoclonal antibody human
IgG
Mammalian
(human cell)
S-RBD protein binding, ELISA validated Picture loading failed.
GMP-V-2019nCoV-S1Ab002 Anti-2019-nCoV Spike (S1 protein) human monoclonal antibody human
IgG
Mammalian
(human cell)
S-RBD protein binding, ELISA validated, Western Blot validated Picture loading failed.

GeneMedi also provides pre-made the lentivirus, adenovirus and AAV vector for the gene ORF plasmids of 2019 nCoV (SARS2 coronavirus):
https://www.genemedi.net/i/2019-ncov-gene-vectors





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