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GeneMedi’s SARS-CoV-2 Pseudotyped virus (PSV) Production
SARS-CoV-2 Pseudovirus-Based Neutralization Assay Service

- Screening your candidates in BSL-2 without live SARS-CoV-2


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For your candidates funtional assay and evaluating:
1) Neutralizing antibodies
2) Peptides blockers (peptide inhibitors)
3) Types of Vaccines (by testing immunized serum from mouse, NHP etc.)
4) Compounds targeting Spike induced cell-fusion.

GeneMedi offers:
1) SARS-CoV-2 Pseudotyped virus packaging and production (Cat.GM-2019nCoV-PSV01)
2) Effector cells: human ACE2 overexpression stable HEK293T cell lines
3) SARS-CoV-2(2019nCoV) Pseudotyped Virus Based Neutralization Assay Service

Click for details of SARS-CoV-2(2019nCoV) Pseudotyped Virus production and assay service.


1. SARS-CoV-2 (2019nCoV) Spike-RBD antigen& Recombinant hACE2 protein

- Competition assay for neutralizing antibodies, peptides inhibitor and vaccines-immunized serums

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Figure. Immobilized 2019-nCoV S Protein RBD-C-His (GMP-V-2019nCoV-SRBD001) can bind 10μg/ml of Human ACE-2-Fc (GMP-H-ACE2002)

Products List:

Cat No. Antigen Name of 2019-nCoV(SARS-CoV-2) Source
(Expression Host)
Tag Bioactivity validation Order
GMP-V-2019nCoV-SRBD001 Recombinant 2019-nCoV(SARS-CoV-2) Spike Protein (RBD, C-His Tag)Picture loading failed. Mamamlian (human cell) C-His ACE2 binding; Picture loading failed.
GMP-V-2019nCoV-SRBD002 Recombinant 2019-nCoV(SARS-CoV-2) Spike Protein (RBD, C-mFC Tag)Picture loading failed. Mamamlian (human cell) C-mFc ACE2 binding; Picture loading failed.
GMP-V-2019nCoV-S1001 Recombinant 2019-nCoV(SARS-CoV-2) Spike Protein (S1, His Tag) Baculovirus-Insect Cells C-His Picture loading failed.
GMP-V-2019nCoV-S1S2001 Recombinant 2019-nCoV(SARS-CoV-2) Spike Protein (S1+S2 ECD, His tag) Baculovirus-Insect Cells C-His Picture loading failed.
GMP-H-ACE2001 Recombinant human soluble ACE2 protein (soluble hACE2,extracellular hACE2,C-His) Mamamlian (human cell) C-His Spike(S-RBD) protein binding Picture loading failed.
GMP-H-ACE2002 Recombinant human soluble ACE2 protein (soluble hACE2, extracellular hACE2, C-FC) Mamamlian (human cell) C-Fc Spike(S-RBD) protein binding Picture loading failed.


2. SARS-CoV-2 (2019nCoV) Non-structure protein (Nsp) Recombinant Antigens

- Recombinant Proteins Of SARS-CoV-2 (2019nCoV) Drugable Target For High-throughput screening (HTS) assay development of antiviral compounds against COVID-19

Name of Non-structure protein of SARS-CoV-2(2019-nCoV, novel coronavirus) Name of Gene in Coronavirus GeneMedi's Recombinant Antigens For Activity Assay High-throughput screening(HTS) assay development information and protocols
(PLpro) papain-like proteinaseNsp3 GMP-V-2019nCoV-PLpro001 Download pdf download
Mpro (main protease,3CLpro)Nsp5 GMP-V-2019nCoV-Mpro001 Download pdf download
Nsp10-CysHis,GFL proteinNsp10 GMP-V-2019nCoV-Nsp10-01 Download pdf download
RNA-dependent RNA polymerase(RdRP)Nsp12 GMP-V-2019nCoV-RdRP001 Download pdf download
2'-O-ribose methyltransferaseNsp16 GMP-V-2019nCoV-Nsp16-01 Download pdf download
Nsp3-X domain(Macro domain)Nsp3 GMP-V-2019nCoV-Nsp3X-01


3. Validated SARS-CoV-2 antibodies: NP antibody and spike antibody


GeneMedi's SARS-CoV-2 NP Antibody Pair And Stability Validation In Sandwich ELISA Picture loading failed.

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Figure. GeneMedi's SARS-CoV2 NP antibody(Nab) pair validation with NP antigen(GMP-V-2019nCoVN002>) in sandwich ELISA.
Nabs were either in PBS solution (stocked in -20 for 7days) or lyophilized (stock at room temperature for 7 days).
GeneMedi's lyophilized antibodies resented excellent stability in the room temperature condition.

Abbreviation Description
Nab001 GMP-V-2019nCoV-NAb001
Nab002 GMP-V-2019nCoV-NAb002
Nab003 GMP-V-2019nCoV-NAb003
Nab004 GMP-V-2019nCoV-NAb004
PBS liquid in PBS buffer
lyophilized lyophilized and stock at room temperature for 7 days before reconstitution
NP SARS-CoV-2 NP atntigen (GMP-V-2019nCoV-N002)
Response Normalized Normalized by Max OD450


Products List:

Cat No. Products Name Host specics Source (Expression Host) Bioactivity validation Order
GMP-V-2019nCoV-NAb001 Anti-2019-nCoV NP human monoclonal antibody human
IgG
Mammalian
(human cell)
N protein binding, ELISA validated as capture antibody and detection antibody.
Pair recommendation with GMP-V-2019nCoV-NAb002 , GMP-V-2019nCoV-NAb003 , GMP-V-2019nCoV-NAb004.
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GMP-V-2019nCoV-NAb002 Anti-2019-nCoV NP human
scFv-Fc antibody
Scfv Mammalian
(human cell)
N protein binding, ELISA validated as capture antibody and detection antibody.
Pair recommendation with GMP-V-2019nCoV-NAb001, GMP-V-2019nCoV-NAb003 , GMP-V-2019nCoV-NAb004.
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GMP-V-2019nCoV-NAb003 Anti-2019-nCoV NP mouse monoclonal antibody(mAb) Mouse
IgG
Hybridoma N protein binding, ELISA validated as capture antibody and detection antibody.
Pair recommendation with GMP-V-2019nCoV-NAb001, GMP-V-2019nCoV-NAb002, GMP-V-2019nCoV-NAb004.
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GMP-V-2019nCoV-NAb004 Anti-2019-nCoV NP mouse monoclonal antibody(mAb) Mouse
IgG
Hybridoma N protein binding, ELISA validated as capture antibody and detection antibody.
Pair recommendation with GMP-V-2019nCoV-NAb001, GMP-V-2019nCoV-NAb002 , GMP-V-2019nCoV-NAb003.
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GMP-V-2019nCoV-S1Ab001 Anti-2019-nCoV Spike (S1 protein) human monoclonal antibody human
IgG
Mammalian
(human cell)
S-RBD protein binding, ELISA validated Picture loading failed.
GMP-V-2019nCoV-S1Ab002 Anti-2019-nCoV Spike (S1 protein) human monoclonal antibody human
IgG
Mammalian
(human cell)
S-RBD protein binding, ELISA validated, Western Blot validated Picture loading failed.

4. Pre-made gene ORF plasmids for SARS-CoV-2 that can be used for mammalian expression or E.coli expression.
   Pre-made lentivirus, adenovirus and AAV vectors for the gene ORF plasmids of SARS-CoV-2.

Non-structure protein(NSP)information of SARS-CoV-2 (2019nCoV)1
2019 nCoV related Gene Description of 2019 nCoV Types of Vectors in option for expression
Spike2019nCoV-Spike protein, Spike S1+S2 protein,
Spike-S1 protein, Spik-RBD protein, coden optimized
Lentiviral, Adenoviral,Mammalian, E.coli
N protein2019nCoV-Nucleocapsid Protein (N protein),coden optimizedMammalian, E.coli
M protein2019nCoV-Membrane protein (M protein), coden optimizedMammalian, E.coli
E proteinpGM-2019nCoV-Envelope Protein (E protein), coden optimizedMammalian, E.coli
ORF82019nCoV-ORF8, coden optimizedMammalian, E.coli
ORF72019nCoV-ORF7a, coden optimizedMammalian, E.coli
ORF62019nCoV-ORF6, coden optimizedMammalian, E.coli
ORF3a2019nCoV-ORF3a, coden optimizedMammalian, E.coli
ORF102019nCoV-ORF10, coden optimizedMammalian, E.coli
ACE2Human, mouseLentiviral, Adenoviral,Mammalian

Click for details of SARS-CoV-2(2019nCoV) genes Pre-made vectors for mammalian and E.coli expression.




References

1. Wu, A. et al. Genome Composition and Divergence of the Novel Coronavirus (2019-nCoV) Originating in China. Cell Host Microbe, doi:10.1016/j.chom.2020.02.001 (2020).




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