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Blog | Gene Therapy Technical Articles

Cre-loxp system and Viral vector (AAV and adenovirus)-based Cre tools (AAV-Cre and Ad-Cre)

Cre-loxP system is widely used in the field of biosciences, especially in the generation of genetically engineered mice (knockout or overexpression), enabling researchers to study the function of gene of interest (GOI).

Products collection for COVID-19 vaccines and drug discovery (neutralizing antibodies, peptides inhibitors or small molecule compounds)

High-throughput screening (HTS) assay development for neutralizing antibodies, peptides, small molecule compounds against COVID-19

Viral vector-based vaccine; DNA-based vaccine; RNA based vaccine - A landscape for vaccine technology against infectious disease, COVID-19 and tumor.

Here we give some basic information on vaccine classification, generation, and application, and make a brief review on the current status of COVID-19 vaccine and tumor vaccine development both in the clinical trial stage and pre-clinical stage.

Landscape Coronavirus Disease 2019 test (COVID-19 test) in vitro -- A comparison of PCR vs Immunoassay vs Crispr-Based test

The outbreak of COVID-19, caused by 2019 novel coronavirus (2019-nCoV), has been a global public health threat and caught the worldwide concern. Scientists throughout the world are sparing all efforts to explore strategies for the determination of the 2019-nCoV virus and diagnosis of COVID-19 rapidly. Several assays are developed for COVID-19 test , including RT-PCR, coronavirus antigens-based immunoassays, and CRISPR-based strategies (Cas13a or Cas12a), etc.

Collection of COVID-19 knonwledge base

GeneMedi produces kinds of recombinant 2019 nCoV(SARS2 coronavirus) antigens as shown below: Nucleocapsid(N protein), Spike(S protein), RBD, S1+S2 ECD, Envelope (E protein), 3C-like Proteinase. All the recombinant proteins of 2019 nCoV are suitable for in functional ELISA,lateral-flow tests, and other immunoassays.

An Insight of comparison between COVID-19 (2019-nCoV) and SARS-CoV in pathology and pathogenesis

COVID-19, a novel pathogenic coronavirus emerged in China and spread globally rapidly. COVID-19 shares above 85% identity in genome with SARS-CoV. Patients infected by COVID-19 and SARS-CoV also reveal similar clinical characteristics. Here we compare the clinical and pathological features between patients infected by COVID-19 and SARS-CoV respectively.

Background of 2019 Wuhan Novel coronavirus - From Wiki

2019 Wuhan Novel coronavirus, (2019-nCoV by WHO), is also known as Wuhan coronavirus, Wuhan seafood market pneumonia virus and Wuhan pneumonia, is a positive-sense, single-stranded RNA coronavirus first reported in 2019. The virus was genomically sequenced after nucleic acid testing on a positive patient sample in a patient with pneumonia during the 2019–2020 Wuhan pneumonia outbreak.

Oncolytic virus immunotherapy (HSV, oncolytic adenovirus) and cancer therapy

Oncolytic virotherapy is a novel therapeutic modality utilizing oncolytic viruses (such as herpes simplex-, pox-, parvo-, or adenoviruses), which selectively replicate in cancer cells, to directly induce tumor cell lysis or indirectly reactivate human immune system to mediate tumor destruction.

CAR-T immunotherapy and cancer therapy

Chimeric antigen receptor (CAR)-modified T cells (CAR-T) are T cells genetically engineered to express CAR, which can specifically recognize their target antigen via the scFv binding domain of CAR, resulting in T cell activation to specifically target and destroy tumor cells.

TCR-T immunotherapy and cancer therapy

T-cell receptor (TCR)–engineered T-cell therapy (TCR-T) is one potentially powerful treatment that utilizes genetically modified natural T cells to specifically target tumors and destroy tumors with greater potentials.

CAR-NK immunotherapy and cancer therapy

Although CAR-T has been applied in clinic, quite a lot of side effects, such as off-target, cytokine release syndrome (CRS) etc., pose a great restriction in their further clinical application.

Immuno-oncology cell therapy

In the past several decades, great progress has been made in the field of anti-tumor therapy, but there are also multiple strategies for tumors to evade the recognition and efficient suppression by the immune system. Therefore, a variety of immunotherapeutic targets and strategies have been developed to reactivate and reorganize the human immune system, such as CAR-T, TCR-T, CAR-NK, DC-CIK, oncolytic virus and so on.

Stem Cell Therapy

Cell therapy based on stem cells is a kind of regenerative medicine with great potential for the treatment of diseases via the differentiation ability or the paracrine effects of stem cells as well as their derivatives.

Genetic re-engineering cell therapy

Besides the immune-oncology therapy and stem cell derived therapy, gene replacement therapy via lentivirus, adenovirus and adenovirus associated virus (AAV) as well as genetic engineered strategies via CRISPR, ZLN, TALEN have been widely applied in the field of cell therapy.

How much do you trust Dr. Google?

Women experiencing signs of breast cancer vary in how they value, use, and trust 'Dr. Google' when making sense of their symptoms, a new study reports.

Weather Forecasting

Weather forecasting is the application of current technology and science to predict the state of the atmosphere for a future time and a given location.

Amino Acid Structure Chart and Reference Table

Amino acids are the backbone of peptides and proteins. All amino acids contain both amino and carboxylic acids, and in certain cases, side chains.

Amino Acid Codes

Amino acids are the backbone of peptides and proteins. All amino acids contain both amino and carboxylic acids, and in certain cases, side chains.