Picture loading failed.

Blog | Gene Therapy Technical Articles

A secret link when Sci-hub is not working

Recently, many readers feel paper prices cannot be afforded, Sci-Hub is a free download this artifact but cannot log in any longer. Will you let them bite people who live it literature? Do not worry! We have found a secret, just to be patient.

Recent "Science" in the article "Who's downloading pirated papers? Everyone", Sci-Hub also discussed the problem of piracy. Some journals while the author of the publication fee income, while readers also received subscription fee, two publishers take the money and do not provide commensurate service. Many authors will encounter when looking for this document.

What is the difference between Ex vivo, In vivo and In vitro?

In vitro (Latin: in glass) studies in experimental biology are those that are conducted using components of an organism that have been isolated from their usual biological surroundings in order to permit a more detailed or more convenient analysis than can be done with whole organisms. Colloquially, these experiments are commonly called "test tube experiments". In contrast, in vivo work is that which is conducted with living organisms in their normal, intact state, while ex vivo studies are conducted on functional organs that have been removed from the intact organism.

Protocol: T7 Endonuclease Assay

Design genomic DNA primers that are approximatley 18 to 22 basepairs in length and have 45-55% GC content. For best results, use primers with a Tm greater than 55 C. Design primers to yield amplicon length between 400-500 bp. In addition, design primers so that the predicted cleavage site is not in the center of the amplicon and the detection reaction will yield two distinct product bands.

Adeno-Associated Virus Gene Therapy Landscape

Adeno-associated viruses (AAV) are virus particles composed of single-stranded DNA surrounded by a protein shell. Despite their simple structure, recombinant AAVs (rAAV) can perform the important role of delivering nucleic acids into cells during gene therapy. Due to the high demand for potentially curative treatments in areas of huge unmet need and the growing availability of AAV vectors, a race to launch successful gene therapy products is underway.

How to achieve accurate and efficient gene transfection of animal target-organs?

Gene Transfection, in layman's words, mainly refers to the increase and decline of gene expression (i.e. gain of function OR loss of function). A classic animal model of gene intervention--genetically engineered mice.Genetically engineered mice included transgenic mice (TG mouse), Knockout mice (KO mouse), and typed mice (KI mouse). In the introduction of CRE-LOXP system, the tissue-specific TG and KO mice were developed.

Protocol for virus injection in vivo

Animal experiment is essential in biomedical research. It contains establishment of a nude mouse tumor model and other disease model. Besides, gene transfection in vivo is included as well.

How much do you trust Dr. Google?

Women experiencing signs of breast cancer vary in how they value, use, and trust 'Dr. Google' when making sense of their symptoms, a new study reports.

Weather Forecasting

Weather forecasting is the application of current technology and science to predict the state of the atmosphere for a future time and a given location.

Amino Acid Structure Chart and Reference Table

Amino acids are the backbone of peptides and proteins. All amino acids contain both amino and carboxylic acids, and in certain cases, side chains.

Amino Acid Codes

Amino acids are the backbone of peptides and proteins. All amino acids contain both amino and carboxylic acids, and in certain cases, side chains.