A consensus sequence (optimally CCA/GCCAUGG) located around the translation start site and involved in its recognition by the ribosome.
Corresponding to the ribosome binding site (RBS) of prokaryotes, the sequence of eukaryotic mRNA located on both sides of the initiation codon AUG is usually ACCACCAUGG) by binding to the translation initiation factor to induce a 5-cap structure. mRNA translation starts, this sequence is called Kozak sequence. Among them, the first base G after AUG and the previous third base A or G are essential for translation initiation; in addition, the 15 bases upstream of AUG generally do not contain T .
Kozak sequence: Kozak consensus sequence, Kozak consensusor Kozak sequence, a sequence that exists in eukaryotic mRNA and plays an important role in the initiation of translation.
The ribosome can recognize this sequence on the mRNA and use it as the translation start site. Note that this sequence is not a ribosomal binding site (RBS) ribosome binding site, but a cap structure.
The best sequence upstream and downstream of the start codon in eukaryotic genes is that the -3 position is a purine base; the +4 position is G, and the A in the start codon AUG is in the +1 position.
A/GCCAUGG is called a kozak sequence or scanning sequence.
KOZAK is a female scientist. She has studied the effects of site-directed mutations around the start codon AUG on transcription and translation, and concluded that in eukaryotes, the sequence at both ends of the start codon is: = G/ N-CIN-C/N-ANNAUGG-, such as GCCACCAUGG, GCCAUGAUGG, has the highest transcription and translation efficiency|Especially the A at position -3 is very important for translation efficiency. This sequence is called Kozak sequence by later generations and is applied The expression vector is under construction.